E01C0658 Human Complement Fragment 3a ELISA kit
The Human Complement Fragment 3a ELISA kit can be used to identify samples from the human species. Complement Fragment 3a can also be called complement C3, complement component 3.
Specifications of Human Complement Fragment 3a ELISA kit
Product Information | |
Cat. No. | E01C0658 |
Product Name | Human Complement Fragment 3a ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 10-250 ng/ml |
Sensitivity | 1.0 ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 10 ng/mL | 1 vial |
STANDARD C (0.5mL) | 25 ng/mL | 1 vial |
STANDARD D (0.5mL) | 50 ng/mL | 1 vial |
STANDARD E (0.5mL) | 100 ng/mL | 1 vial |
STANDARD F (0.5mL) | 250 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
C3a ELISA kit uses an anti-C3a antibody and an C3a-HRP conjugate in a competitive enzyme immunoassay method. C3a-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-C3a antibody binding site between C3a from samples and C3a-HRP conjugate, the intensity of the color is inversely proportional to the concentration of C3a. Since the number of sites is limited, as more sites are occupied by C3a from the sample, fewer sites are left to bind C3a-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The C3a concentration in each sample is interpolated from this standard curve. |
Quality Control on Human Complement Fragment 3a ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between C3a and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Human Complement Fragment 3a ELISA kit
Citations of Human Complement Fragment 3a ELISA kit
E01C0658 has been referenced in the below publications：
Mild hypothermia inhibits systemic and cerebral complement activation in a swine model of cardiac arrest.
