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BlueGene Biotech Human Hyaluronic Acid ELISA kit

E01H0029 Human Hyaluronic Acid ELISA kit

The Human Hyaluronic Acid ELISA kit can be used to identify samples from the human species. Hyaluronic Acid can also be called HA, hyaluronan, Hyaluronan, Hyaluronate.

Specifications of Human Hyaluronic Acid ELISA kit

Product Information

Cat. No.

E01H0029 

Product Name

Human Hyaluronic Acid ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

25-500 ng/ml

Sensitivity

1.0 ng/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

25 ng/mL

1 vial

STANDARD C (0.5mL)

50 ng/mL

1 vial

STANDARD D (0.5mL)

100 ng/mL

1 vial

STANDARD E (0.5mL)

250 ng/mL

1 vial

STANDARD F (0.5mL)

500 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

HA ELISA kit uses an anti-HA antibody and an HA-HRP conjugate in a competitive enzyme immunoassay method. HA-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-HA antibody binding site between HA from samples and HA-HRP conjugate, the intensity of the color is inversely proportional to the concentration of HA. Since the number of sites is limited, as more sites are occupied by HA from the sample, fewer sites are left to bind HA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HA concentration in each sample is interpolated from this standard curve.


Quality Control on Human Hyaluronic Acid ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between HA and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Human Hyaluronic Acid ELISA kit

Summary of the Assay Procedure for Human Hyaluronic Acid ELISA kit

Citations of Human Hyaluronic Acid ELISA kit

E01H0029 has been referenced in the below publications:

Cartilage turnover and intra-articular corticosteroid injections in knee osteoarthritis.

Flavonoid-rich Scabiosa comosa inflorescence extract attenuates CCl4-induced hepatic fibrosis by modulating TGF-β-induced Smad3 phosphorylation.

Effects of Complementary Combination Therapy of Korean Red Ginseng and Antiviral Agents in Chronic Hepatitis B.

Cartilage metabolic markers and antioxidants: its correlation in osteoarthritic patients of sikkim.

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