E01I0309 Human Interleukin 17 ELISA kit
The Human Interleukin 17 ELISA kit can be used to identify samples from the human species. Interleukin 17 can also be called IL17, IL 17, IL-17, Interleukin-17.
Human Interleukin 17 ELISA kit
48 Tests / 96 Tests
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
STANDARD A (0.5mL)
STANDARD B (0.5mL)
STANDARD C (0.5mL)
STANDARD D (0.5mL)
STANDARD E (0.5mL)
STANDARD F (0.5mL)
WASH SOLUTION (100 x)
Principle of the Assay
IL17 ELISA kit uses an anti-IL17 antibody and a IL17-HRP conjugate in a competitive enzyme immunoassay method. IL17-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-IL17 antibody binding site between IL17 from samples and IL17-HRP conjugate, the intensity of the color is inversely proportional to the concentration of IL17. Since the number of sites is limited, as more sites are occupied by IL17 from the sample, fewer sites are left to bind IL17-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL17 concentration in each sample is interpolated from this standard curve.
Coefficient of Variance
Intra Variation% ＜10%
Inter Variation% ＜12%
No significant cross-reactivity or interference between IL17 and analogues was observed.
E01I0309 has been referenced in the below publications：
Clinical Evaluation of NB-UVB Combined with Tuibai Decoction for Treating Vitiligo Vulgaris in 59 Cases.
Effect of Yangyin Yifei Fang on Leukotrience B4 Level in Patients with Chronic Obstructive Pulmonary Disease in Stable Stage of Qi and Yin Deficiency Type.
Expression and clinical significance of serum IL-17 and IL-22 in patients with rheumatoid arthritis.
The Effects and Mechanisms of IL-17A in degenerated Lumbar disc inflammative reaction and cartilage degeneration in ONFH.
Study of cytokines and chest chest high-resolution CT, pulmonary function on children patients with Mycoplasma pneumoniae pneumonia.
The serum levels of IL-17 and IL-23 in children with Mycoplasma pneumoniae pneumonia.
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