E01K0078 Human Kallikrein 3 ELISA ki
The Human Kallikrein 3 ELISA ki can be used to identify samples from the human species. Kallikrein 3 can also be called KLK3, APS, KLK2A1, HK3, KLKB1, Seminin, Prostate Specific Antigen, Kallikrein-Related Peptidase 3, Semenogelase, g-Seminoprotein, P-30 Antigen, Plasma kallikrein.
Product Information | |
Cat. No. | E01K0078 |
Product Name | Human Kallikrein 3 ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 100-2500 pg/ml |
Sensitivity | 1.0 pg/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 100 pg/mL | 1 vial |
STANDARD C (0.5mL) | 250 pg/mL | 1 vial |
STANDARD D (0.5mL) | 500 pg/mL | 1 vial |
STANDARD E (0.5mL) | 1000 pg/mL | 1 vial |
STANDARD F (0.5mL) | 2500 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
| KLK3 ELISA kit uses an anti-KLK3 antibody and an KLK3-HRP conjugate in a competitive enzyme immunoassay method. KLK3-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-KLK3 antibody binding site between KLK3 from samples and KLK3-HRP conjugate, the intensity of the color is inversely proportional to the concentration of KLK3. Since the number of sites is limited, as more sites are occupied by KLK3 from the sample, fewer sites are left to bind KLK3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The KLK3 concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between KLK3 and analogues was observed. | |
E11I0308 has been referenced in the below publications：
Infection of Cattle With Cryptosporidium parvum: Mast Cell Accumulation in Small Intestine Mucosa.
To separation of lactic acid bacteria and yeast cultures for the application of cow and newborn calf perinatal care.
Cryptosporidium parvum. Protective Immunity against Cryptosporidiosis with Recombinant Lactobacillus Live Vaccines Expressing cp966 after Oral Immunization.
Effects of complex anionic salt premix on production performance and health stadus of perinatal cows.
蒲公英水提物对断奶仔猪免疫功能和血清抗氧化功能的效应试验。
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