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  • bovine serum albumin elisa 1
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BlueGene Biotech Human Xanthine oxidase ELISA kit (E01X0001)

E01X0001 Human Xanthine oxidase ELISA kit

The Human Xanthine oxidase ELISA kit can be used to identify samples from the human species. Xanthine oxidase can also be called XO, XAO.

Products

Specifications of Bovine Interleukin 2 ELISA kit

Product Information

Cat. No.

E01X0001

Product Name

Human Xanthine oxidase ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

2.5-50 U/L

Sensitivity

0.1 U/L

Principal

Sandwich ELISA

Sample Volume

50 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 U/L

1 vial

STANDARD B (0.5mL)

2.5 U/L

1 vial

STANDARD C (0.5mL)

5.0 U/L

1 vial

STANDARD D (0.5mL)

10 U/L

1 vial

STANDARD E (0.5mL)

25 U/L

1 vial

STANDARD F (0.5mL)

50 U/L

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

XOD ELISA kit uses an anti-XOD antibody and an XOD-HRP conjugate in a competitive enzyme immunoassay method. XOD-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-XOD antibody binding site between XOD from samples and XOD-HRP conjugate, the intensity of the color is inversely proportional to the concentration of XOD. Since the number of sites is limited, as more sites are occupied by XOD from the sample, fewer sites are left to bind XOD-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The XOD concentration in each sample is interpolated from this standard curve.


Quality Control on Human Xanthine oxidase ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between XOD and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Human Xanthine oxidase ELISA kit

Summary of the Assay Procedure for Human Xanthine oxidase ELISA kit

Citations of Human Xanthine oxidase ELISA kit

E01X0001 has been referenced in the below publications:

Antioxidant Blueberry Anthocyanins Induce Vasodilation via PI3K/Akt Signaling Pathway in High-Glucose-Induced Human Umbilical Vein Endothelial Cells.


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