E01X0001 Human Xanthine oxidase ELISA kit
The Human Xanthine oxidase ELISA kit can be used to identify samples from the human species. Xanthine oxidase can also be called XO, XAO.
Specifications of Bovine Interleukin 2 ELISA kit
Product Information | |
Cat. No. | E01X0001 |
Product Name | Human Xanthine oxidase ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 2.5-50 U/L |
Sensitivity | 0.1 U/L |
Principal | Sandwich ELISA |
Sample Volume | 50 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 U/L | 1 vial |
STANDARD B (0.5mL) | 2.5 U/L | 1 vial |
STANDARD C (0.5mL) | 5.0 U/L | 1 vial |
STANDARD D (0.5mL) | 10 U/L | 1 vial |
STANDARD E (0.5mL) | 25 U/L | 1 vial |
STANDARD F (0.5mL) | 50 U/L | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
XOD ELISA kit uses an anti-XOD antibody and an XOD-HRP conjugate in a competitive enzyme immunoassay method. XOD-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-XOD antibody binding site between XOD from samples and XOD-HRP conjugate, the intensity of the color is inversely proportional to the concentration of XOD. Since the number of sites is limited, as more sites are occupied by XOD from the sample, fewer sites are left to bind XOD-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The XOD concentration in each sample is interpolated from this standard curve. |
Quality Control on Human Xanthine oxidase ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between XOD and analogues was observed. | |
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Summary of the Assay Procedure for Human Xanthine oxidase ELISA kit
Citations of Human Xanthine oxidase ELISA kit
E01X0001 has been referenced in the below publications：
Antioxidant Blueberry Anthocyanins Induce Vasodilation via PI3K/Akt Signaling Pathway in High-Glucose-Induced Human Umbilical Vein Endothelial Cells.
