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BlueGene Biotech Human Apolipoprotein M ELISA kit

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E01A0522 Human Apolipoprotein M ELISA kit

The Human Apolipoprotein M ELISA kit can be used to identify samples from the human species. Apolipoprotein M can also be called  G3a, HSPC336, NG20, apo-M.

Specifications of Human Apolipoprotein M ELISA kit

Product Information

Cat. No.

E01A0522

Product Name

Human Apolipoprotein M ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

10-250 ng/ml

Sensitivity

1.0 ng/ml

Principal

Sandwich ELISA

Sample Volume

50 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

10.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

10 ng/mL

1 vial

STANDARD C (0.5mL)

25 ng/mL

1 vial

STANDARD D (0.5mL)

50 ng/mL

1 vial

STANDARD E (0.5mL)

100 ng/mL

1 vial

STANDARD F (0.5mL)

250 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

APOM ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-APOM antibody and an APOM-HRP conjugate. The assay sample and buffer are incubated together with APOM-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the APOM concentration since APOM from samples and APOM-HRP conjugate compete for the anti-APOM antibody binding site. Since the number of sites is limited, as more sites are occupied by APOM from the sample, fewer sites are left to bind APOM-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The APOM concentration in each sample is interpolated from this standard curve. 


Quality Control on Human Apolipoprotein M ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between APOM and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Human Apolipoprotein M ELISA kit

Summary of the Assay Procedure for Human Apolipoprotein M ELISA kit

Citations of Human Apolipoprotein M ELISA kit

E01A0522 has been referenced in the below publications:

Effect of Extended-Release Niacin on High-Density Lipoprotein (HDL) Functionality, Lipoprotein Metabolism, and Mediators of Vascular Inflammation in Statin-Treated Patients.

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