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BlueGene Biotech Human NAD(P)H dehydrogenase [quinone] 1 ELISA kit

E01N0670 Human NAD(P)H dehydrogenase [quinone] 1 ELISA kit

The Human NAD(P)H dehydrogenase [quinone] 1 ELISA kit can be used to identify samples from the human species. NAD(P)H dehydrogenase [quinone] 1 can also be called NQO1, DHQU, DIA4, DTD, NMOR1, NMORI, QR1, Azoreductase, DT-diaphorase, Menadione reductase, Phylloquinone reductase, Quinone reductase 1, NAD(P)H:quinone oxidoreductase 1, DT-diaphorase 1 Publication.

Specifications of Human NAD(P)H dehydrogenase [quinone] 1 ELISA kit

Product Information

Cat. No.

E01N0670

Product Name

Human NAD(P)H dehydrogenase [quinone] 1 ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

1.0-25 ng/ml

Sensitivity

0.1 ng/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

1.0 ng/mL

1 vial

STANDARD C (0.5mL)

2.5 ng/mL

1 vial

STANDARD D (0.5mL)

5.0 ng/mL

1 vial

STANDARD E (0.5mL)

10 ng/mL

1 vial

STANDARD F (0.5mL)

25 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

NQO1 ELISA kit uses an anti-NQO1 antibody and an NQO1-HRP conjugate in a competitive enzyme immunoassay method. NQO1-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-NQO1 antibody binding site between NQO1 from samples and NQO1-HRP conjugate, the intensity of the color is inversely proportional to the concentration of NQO1. Since the number of sites is limited, as more sites are occupied by NQO1 from the sample, fewer sites are left to bind NQO1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NQO1 concentration in each sample is interpolated from this standard curve.


Quality Control on Human NAD(P)H dehydrogenase [quinone] 1 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between NQO1 and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Human NAD(P)H dehydrogenase [quinone] 1 ELISA kit

Summary of the Assay Procedure for Human NAD(P)H dehydrogenase [quinone] 1 ELISA kit

Citations of Human NAD(P)H dehydrogenase [quinone] 1 ELISA kit

E01N0670 has been referenced in the below publications:

To investigate the association between distribution of body fat and coronary heart disease(CHD)by analysing the correlation between body fat distribution measures and incidence,type and severity of coronary heart disease.

Pilot study of bevacizumab in combination with docetaxel and cyclophosphamide as adjuvant treatment for patients with early stage HER-2 negative breast cancer, including analysis of candidate circulating markers of cardiac toxicity: ICORG 08-10 trial.

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