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  • bovine serum albumin elisa 1

BlueGene Biotech Human Karyopherin Alpha 2 ELISA kit

E01K0096 Human Karyopherin Alpha 2 ELISA kit

The Human Karyopherin Alpha 2 ELISA kit can be used to identify samples from the human species. Karyopherin Alpha 2 can also be called IPOA1, QIP2, RCH1, SRP1alpha, RAG Cohort 1, Importin Alpha 1, RAG cohort protein 1, SRP1-alpha.

Specifications of Human Karyopherin Alpha 2 ELISA kit

Product Information

Cat. No.

E01K0096

Product Name

Human Karyopherin Alpha 2 ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

5.0-100 ng/ml

Sensitivity

1.0 ng/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

5.0 ng/mL

1 vial

STANDARD C (0.5mL)

10 ng/mL

1 vial

STANDARD D (0.5mL)

25 ng/mL

1 vial

STANDARD E (0.5mL)

50 ng/mL

1 vial

STANDARD F (0.5mL)

100 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

KPNa2 ELISA kit uses an anti-KPNa2 antibody and an KPNa2-HRP conjugate in a competitive enzyme immunoassay method. KPNa2-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-KPNa2 antibody binding site between KPNa2 from samples and KPNa2-HRP conjugate, the intensity of the color is inversely proportional to the concentration of KPNa2. Since the number of sites is limited, as more sites are occupied by KPNa2 from the sample, fewer sites are left to bind KPNa2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The KPNa2 concentration in each sample is interpolated from this standard curve.


Quality Control on Human Karyopherin Alpha 2 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between KPNa2 and analogues was observed.


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Summary of the Assay Procedure for Human Karyopherin Alpha 2 ELISA kit

Summary of the Assay Procedure for Human Karyopherin Alpha 2 ELISA kit

Citations of Human Karyopherin Alpha 2 ELISA kit

E01K0096 has been referenced in the below publications:

Karyopherin alpha 2 expression is a novel diagnostic and prognostic factor for colorectal cancer.

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