E01K0096 Human Karyopherin Alpha 2 ELISA kit
The Human Karyopherin Alpha 2 ELISA kit can be used to identify samples from the human species. Karyopherin Alpha 2 can also be called IPOA1, QIP2, RCH1, SRP1alpha, RAG Cohort 1, Importin Alpha 1, RAG cohort protein 1, SRP1-alpha.
Product Information | |
Cat. No. | E01K0096 |
Product Name | Human Karyopherin Alpha 2 ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 5.0-100 ng/ml |
Sensitivity | 1.0 ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD C (0.5mL) | 10 ng/mL | 1 vial |
STANDARD D (0.5mL) | 25 ng/mL | 1 vial |
STANDARD E (0.5mL) | 50 ng/mL | 1 vial |
STANDARD F (0.5mL) | 100 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
KPNa2 ELISA kit uses an anti-KPNa2 antibody and an KPNa2-HRP conjugate in a competitive enzyme immunoassay method. KPNa2-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-KPNa2 antibody binding site between KPNa2 from samples and KPNa2-HRP conjugate, the intensity of the color is inversely proportional to the concentration of KPNa2. Since the number of sites is limited, as more sites are occupied by KPNa2 from the sample, fewer sites are left to bind KPNa2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The KPNa2 concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between KPNa2 and analogues was observed. | |
E01K0096 has been referenced in the below publications：
Karyopherin alpha 2 expression is a novel diagnostic and prognostic factor for colorectal cancer.
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