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BlueGene Biotech Human Phosphatidylinositol 4 Kinase Catalytic Alpha ELISA kit

E01P0660 Human Phosphatidylinositol 4 Kinase Catalytic Alpha ELISA kit

The Human Phosphatidylinositol 4 Kinase Catalytic Alpha ELISA kit can be used to identify samples from the human species. Phosphatidylinositol 4 Kinase Catalytic Alpha can also be called PI4Ka, PI4KA, PI4K-ALPHA, PIK4CA, pi4K230, PMGYCHA, phosphatidylinositol 4-kinase alpha, SPG84, GIDID2, PI4-kinase alpha, PI4K-alpha, PtdIns-4-kinase alpha, PI4-kinase alpha, PI4K-alpha, PtdIns-4-kinase alpha.

Specifications of Human Phosphatidylinositol 4 Kinase Catalytic Alpha ELISA kit

Product Information

Cat. No.

E01P0660

Product Name

Human Phosphatidylinositol 4 Kinase Catalytic Alpha ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

1.0-25 ng/ml

Sensitivity

0.1 ng/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

1.0 ng/mL

1 vial

STANDARD C (0.5mL)

2.5 ng/mL

1 vial

STANDARD D (0.5mL)

5.0 ng/mL

1 vial

STANDARD E (0.5mL)

10 ng/mL

1 vial

STANDARD F (0.5mL)

25 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

PI4Ka ELISA kit uses an anti-PI4Ka antibody and an PI4Ka-HRP conjugate in a competitive enzyme immunoassay method. PI4Ka-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-PI4Ka antibody binding site between PI4Ka from samples and PI4Ka-HRP conjugate, the intensity of the color is inversely proportional to the concentration of PI4Ka. Since the number of sites is limited, as more sites are occupied by PI4Ka from the sample, fewer sites are left to bind PI4Ka-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PI4Ka concentration in each sample is interpolated from this standard curve.


Quality Control on Human Phosphatidylinositol 4 Kinase Catalytic Alpha ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between PI4Ka and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Human Phosphatidylinositol 4 Kinase Catalytic Alpha ELISA kit

Summary of the Assay Procedure for Human Phosphatidylinositol 4 Kinase Catalytic Alpha ELISA kit

Citations of Human Phosphatidylinositol 4 Kinase Catalytic Alpha ELISA kit

E01P0660 has been referenced in the below publications:

Correlation between expression of PI4KA in serum and HVB DNA load in patients with chronic hepatitis B.

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