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BlueGene Biotech Human Soluble tumor necrosis factor-related apoptosis inducing ligand ELISA (E01S0295)

E01S0295 Human Soluble tumor necrosis factor-related apoptosis inducing ligand ELISA

The Human Soluble tumor necrosis factor-related apoptosis inducing ligand ELISA can be used to identify samples from the human species. Soluble tumor necrosis factor-related apoptosis inducing ligand can also be called sTNFSF10, sAPO2L, sApo-2L, sCD253, TL2, sTRAIL, sTNLG6A, stumor necrosis factor superfamily member 10, sTNF superfamily member 10.

Products

Specifications of Human Soluble tumor necrosis factor-related apoptosis inducing ligand ELISA

Product Information

Cat. No.

E01S0295

Product Name

Human Soluble tumor necrosis factor-related apoptosis inducing ligand ELISA

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

100-2500 pg/ml

Sensitivity

1.0 pg/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

100 pg/mL

1 vial

STANDARD C (0.5mL)

250 pg/mL

1 vial

STANDARD D (0.5mL)

500 pg/mL

1 vial

STANDARD E (0.5mL)

1000 pg/mL

1 vial

STANDARD F (0.5mL)

2500 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

Strail ELISA kit uses an anti-Strail antibody and an Strail-HRP conjugate in a competitive enzyme immunoassay method. Strail-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-Strail antibody binding site between Strail from samples and Strail-HRP conjugate, the intensity of the color is inversely proportional to the concentration of Strail. Since the number of sites is limited, as more sites are occupied by Strail from the sample, fewer sites are left to bind Strail-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Strail concentration in each sample is interpolated from this standard curve.


Quality Control on Human Soluble tumor necrosis factor-related apoptosis inducing ligand ELISA

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between Strail and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Human Soluble tumor necrosis factor-related apoptosis inducing ligand ELISA

Summary of the Assay Procedure for Human Soluble tumor necrosis factor-related apoptosis inducing ligand ELISA

Citations of Human Soluble tumor necrosis factor-related apoptosis inducing ligand ELISA

E01S0295 has been referenced in the below publications:

Effects of Entecavir on serum sTRAIL in patients with hepatitis B virus-related cirrhosis.

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