En
Email
  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Human Tumor Necrosis Factor Alpha ELISA kit (E01T0008)

E01T0008 Human Tumor Necrosis Factor Alpha ELISA kit

The Human Tumor Necrosis Factor Alpha ELISA kit can be used to identify samples from the human species. Tumor Necrosis Factor Alpha can also be called TNFa, TNFAlpha, DIF, TNF-A, TNFSF2, Cachectin, Tumor Necrosis Factor Ligand Superfamily Member 2.

Products

Specifications of Human Tumor Necrosis Factor Alpha ELISA kit

Product Information

Cat. No.

E01T0008

Product Name

Human Tumor Necrosis Factor Alpha ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

50-1000 pg/ml

Sensitivity

1.0 pg/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

50 pg/mL

1 vial

STANDARD C (0.5mL)

100 pg/mL

1 vial

STANDARD D (0.5mL)

250 pg/mL

1 vial

STANDARD E (0.5mL)

500 pg/mL

1 vial

STANDARD F (0.5mL)

1000 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

TNFAlpha ELISA kit uses an anti-TNFAlpha antibody and an TNFAlpha-HRP conjugate in a competitive enzyme immunoassay method. TNFAlpha-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-TNFAlpha antibody binding site between TNFAlpha from samples and TNFAlpha-HRP conjugate, the intensity of the color is inversely proportional to the concentration of TNFAlpha. Since the number of sites is limited, as more sites are occupied by TNFAlpha from the sample, fewer sites are left to bind TNFAlpha-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TNFAlpha concentration in each sample is interpolated from this standard curve.


Quality Control on Human Tumor Necrosis Factor Alpha ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between TNFAlpha and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Human Tumor Necrosis Factor Alpha ELISA kit

Summary of the Assay Procedure for Human Tumor Necrosis Factor Alpha ELISA kit

Citations of Human Tumor Necrosis Factor Alpha ELISA kit

E01T0008 has been referenced in the below publications:

Hydrogen-rich water decreases serum low-density lipoprotein cholesterol levels and improves high-density lipoprotein function in patients with potential metabolic syndrome.

Mild hypothermia inhibits systemic and cerebral complement activation in a swine model of cardiac arrest.

Synthesis and cardiovascular protective effects of quercetin 7-O-sialic acid.

Dysregulated hepatic bile acids collaboratively promote liver carcinogenesis.

Mesenchymal Stem Cells Stabilize Atherosclerotic Vulnerable Plaque by Anti-Inflammatory Properties.

Chronic Noise Exposure Acts Cumulatively to Exacerbate Alzheimer’s Disease-Like Amyloid-β Pathology and Neuroinflammation in the Rat Hippocampus.

High-density lipoprotein inhibits ox-LDL-induced adipokine secretion by upregulating SR-BI expression and suppressing ER Stress pathway.

Related Bluegene Biotech Products

Join us and become BlueGene's distributor, you will get a broad range of Life science research products and more than 12 years of experienced technical supports.