E01G0013 Human Ghrelin ELISA kit
The Human Ghrelin ELISA kit can be used to identify samples from the human species. Ghrelin can also be called Appetite regulating hormone, Ghrelin 27, Ghrelin 28, Ghrelin/obestatin prepropeptide, Growth hormone releasing peptide, Growth hormone secretagogue, M46 protein, Motilin related peptide, MTLRP, Obestatin, Obestatin preprohormone.
Product Information | |
Cat. No. | E01G0013 |
Product Name | Human Ghrelin ELISA kit |
Species | Human |
Product Size | 48 Tests / 96 Tests |
Concentration | 5.0-100 ng/mL |
Sensitivity | 1.0 ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD C (0.5mL) | 10 ng/mL | 1 vial |
STANDARD D (0.5mL) | 25 ng/mL | 1 vial |
STANDARD E (0.5mL) | 50 ng/mL | 1 vial |
STANDARD F (0.5mL) | 100 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
Ghrelin ELISA kit uses an anti-Ghrelin antibody and an Ghrelin-HRP conjugate in a competitive enzyme immunoassay method. Ghrelin-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-Ghrelin antibody binding site between Ghrelin from samples and Ghrelin-HRP conjugate, the intensity of the color is inversely proportional to the concentration of Ghrelin. Since the number of sites is limited, as more sites are occupied by Ghrelin from the sample, fewer sites are left to bind Ghrelin-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Ghrelin concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between Ghrelin and analogues was observed. | |
E01G0013 has been referenced in the below publications：
Effect of amitriptyline on gastrointestinal function and brain-gut peptides: A double-blind trial.
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