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BlueGene Biotech Mouse Anti-OVA-IgE ELISA kit

E03O0334 Mouse Anti-OVA-IgE ELISA kit

The Mouse Anti-OVA-IgE ELISA kit can be used to identify samples from the mouse species. Anti-OVA-IgE can also be called OVA IgE.

Specifications of Mouse Anti-OVA-IgE ELISA kit

Product Information

Cat. No.

E03O0334

Product Name

Mouse Anti-OVA-IgE ELISA kit

Species

Mouse

Product Size

48 Tests / 96 Tests

Concentration

1.0-25μg/ml

Sensitivity

0.1μg/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ug/ml1 vial

STANDARD B (0.5mL)

1.0 ug/ml

1 vial

STANDARD C (0.5mL)

2.5 ug/ml

1 vial

STANDARD D (0.5mL)

5.0 ug/ml

1 vial

STANDARD E (0.5mL)

10 ug/ml

1 vial

STANDARD F (0.5mL)

25 ug/ml

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

OVA IgE ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-OVA IgE antibody and an OVA IgE-HRP conjugate. The assay sample and buffer are incubated together with OVA IgE-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the OVA IgE concentration since OVA IgE from samples and OVA IgE-HRP conjugate compete for the anti-OVA IgE antibody binding site. Since the number of sites is limited, as more sites are occupied by OVA IgE from the sample, fewer sites are left to bind OVA IgE-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The OVA IgE concentration in each sample is interpolated from this standard curve. 


Quality Control on Mouse Anti-OVA-IgE ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between OVA IgE and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Mouse Anti-OVA-IgE ELISA kit

Summary of the Assay Procedure for Mouse Anti-OVA-IgE ELISA kit

Citations of Mouse Anti-OVA-IgE ELISA kit

E03O0334 has been referenced in the below publications:

Downregulation of Orai1 expression in the airway alleviates murine allergic rhinitis.

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