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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Mouse Cardiac Troponin T ELISA kit

E03T0017 Mouse Cardiac Troponin T ELISA kit

The Mouse Cardiac Troponin T ELISA kit can be used to identify samples from the mouse species. Cardiac Troponin T can cTnT, Cardiac muscle troponin T, CMH2, TnTc, CardiomyopathyHypertrophic 2, CMD1D, CMPD2.

Specifications of Mouse Cardiac Troponin T ELISA kit

Product Information

Cat. No.

E03T0017

Product Name

Mouse Cardiac Troponin T ELISA kit

Species

Mouse

Product Size

48 Tests / 96 Tests

Concentration

1.0-25ng/ml

Sensitivity

0.1ng/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/ml1 vial

STANDARD B (0.5mL)

1.0 ng/ml

1 vial

STANDARD C (0.5mL)

2.5 ng/ml

1 vial

STANDARD D (0.5mL)

5.0 ng/ml

1 vial

STANDARD E (0.5mL)

10 ng/ml

1 vial

STANDARD F (0.5mL)

25 ng/ml

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

cTnT ELISA kit uses an anti-cTnT antibody and an cTnT-HRP conjugate in a competitive enzyme immunoassay method. cTnT-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-cTnT antibody binding site between cTnT from samples and cTnT-HRP conjugate, the intensity of the color is inversely proportional to the concentration of cTnT. Since the number of sites is limited, as more sites are occupied by cTnT from the sample, fewer sites are left to bind cTnT-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The cTnT concentration in each sample is interpolated from this standard curve.


Quality Control on Mouse Cardiac Troponin T ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between cTnT and analogues was observed.


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Summary of the Assay Procedure for Mouse Cardiac Troponin T ELISA kit

Summary of the Assay Procedure for Mouse Cardiac Troponin T ELISA kit

Citations of Mouse Cardiac Troponin T ELISA kit

E03T0017 has been referenced in the below publications:

Influence on proliferation and inducing capacity of bone mesenchymal stem cells during myocardial differentiation and construction of engineered myocardium-like tissue in vitro.


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