E03C0549 Mouse Caspase 3 ELISA kit
The Mouse Caspase 3 ELISA kit can be used to identify samples from the mouse species. Caspase 3 can also be called CPP32, CPP32B, SCA-1, caspase 3, CASP3．
Specifications of Mouse Caspase 3 ELISA kit
Product Information | |
Cat. No. | E03C0549 |
Product Name | Mouse Caspase 3 ELISA kit |
Species | Mouse |
Product Size | 48 Tests / 96 Tests |
Concentration | 0.5-10ng/ml |
Sensitivity | 0.1ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/ml | 1 vial |
STANDARD B (0.5mL) | 0.5 ng/ml | 1 vial |
STANDARD C (0.5mL) | 1.0 ng/ml | 1 vial |
STANDARD D (0.5mL) | 2.5 ng/ml | 1 vial |
STANDARD E (0.5mL) | 5.0ng/ml | 1 vial |
STANDARD F (0.5mL) | 10 ng/ml | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
CASP3 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-CASP3 antibody and a CASP3-HRP conjugate. The assay sample and buffer are incubated together with CASP3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CASP3 concentration since CASP3 from samples and CASP3-HRP conjugate compete for the anti-CASP3 antibody binding site. Since the number of sites is limited, as more sites are occupied by CASP3 from the sample, fewer sites are left to bind CASP3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CASP3 concentration in each sample is interpolated from this standard curve. |
Quality Control on Mouse Caspase 3 ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between CASP3 and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Mouse Caspase 3 ELISA kit
Citations of Mouse Caspase 3 ELISA kit
E03C0549 has been referenced in the below publications：
Effects of matrine on the proliferation and apoptosis of human medulloblastoma cell line D341.
Study on the Lung Damage in Mice Induced by a Combined Exposure of PM2.5 and Formaldehyde and its Molecular Mechanism.
Quercetin and lithium chloride potentiate the protective effects of carvedilol against renal ischemia-reperfusion injury in high-fructose, high-fat diet-fed Swiss albino mice independent of renal lipid signaling.
Carvedilol protects against hepatic ischemia/reperfusion injury in high-fructose/high-fat diet-fed mice: Role of G protein-coupled receptor kinase 2 and 5.
