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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Mouse Cluster of Differentiation 8 ELISA kit

E03C0007 Mouse Cluster of Differentiation 8 ELISA kit

The Mouse Cluster of Differentiation 8 ELISA kit can be used to identify samples from the mouse species. Cluster of Differentiation 8 can also be called CD8, P32, CD8-A, Leu2, MAL, T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2.

Specifications of Mouse Cluster of Differentiation 8 ELISA kit

Product Information

Cat. No.

E03C0007

Product Name

Mouse Cluster of Differentiation 8 ELISA kit

Species

Mouse

Product Size

48 Tests / 96 Tests

Concentration

0.5-10ng/ml

Sensitivity

0.1ng/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/ml1 vial

STANDARD B (0.5mL)

0.5 ng/ml

1 vial

STANDARD C (0.5mL)

1.0 ng/ml

1 vial

STANDARD D (0.5mL)

2.5 ng/ml

1 vial

STANDARD E (0.5mL)

5.0ng/ml

1 vial

STANDARD F (0.5mL)

10 ng/ml

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

CD8 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-CD8 antibody and a CD8-HRP conjugate. The assay sample and buffer are incubated together with CD8-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CD8 concentration since CD8 from samples and CD8-HRP conjugate compete for the anti-CD8 antibody binding site. Since the number of sites is limited, as more sites are occupied by CD8 from the sample, fewer sites are left to bind CD8-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CD8 concentration in each sample is interpolated from this standard curve. 


Quality Control on Mouse Cluster of Differentiation 8 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between CD8 and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Mouse Cluster of Differentiation 8 ELISA kit

Summary of the Assay Procedure for Mouse Cluster of Differentiation 8 ELISA kit

Citations of Mouse Cluster of Differentiation 8 ELISA kit

E03C0007 has been referenced in the below publications:

Protection and immunoregulation of Zhongsheng Pill on mice infected with H1N1 virus.

Study of Regulating Effect of Seriphidium Decoction on Immunologic Function of Normal Mice.


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