E03I0332 Mouse Insulin-Degrading Enzyme ELISA kit
The Mouse Insulin-Degrading Enzyme ELISA kit can be used to identify samples from the mouse species. Insulin-Degrading Enzyme can also be called Insulysin, Insulin Protease, Abeta-degrading protease, Insulinase, IDE.
Product Information | |
Cat. No. | E03I0332 |
Product Name | Mouse Insulin-Degrading Enzyme ELISA kit |
Species | Mouse |
Product Size | 48 Tests / 96 Tests |
Concentration | 1.0-25ng/ml |
Sensitivity | 0.1ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/ml | 1 vial |
STANDARD B (0.5mL) | 1.0 ng/ml | 1 vial |
STANDARD C (0.5mL) | 2.5 ng/ml | 1 vial |
STANDARD D (0.5mL) | 5.0 ng/ml | 1 vial |
STANDARD E (0.5mL) | 10 ng/ml | 1 vial |
STANDARD F (0.5mL) | 25 ng/ml | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
IDE ELISA kit uses an anti-IDE antibody and an IDE-HRP conjugate in a competitive enzyme immunoassay method. IDE-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-IDE antibody binding site between IDE from samples and IDE-HRP conjugate, the intensity of the color is inversely proportional to the concentration of IDE. Since the number of sites is limited, as more sites are occupied by IDE from the sample, fewer sites are left to bind IDE-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IDE concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between IDE and analogues was observed. | |
E03I0332 has been referenced in the below publications：
Effect of Different Factors of Moxibustion on P13K／AKT Signal Pathway and Cortical 6-amyioid Protein Precipitation in Brain Tissue of Alzheimer Mice.
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