En
Email
  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Mouse Collagen Type I ELISA kit

E03C0014 Mouse Collagen Type I ELISA kit

The Mouse Collagen Type I ELISA kit can be used to identify samples from the mouse species. Collagen Type I can also be called Collagen type I, Alpha 1 type I collagen, COL1A1, Collagen I alpha 1 polypeptide, Collagen Of Skin Tendon And Bone, Collagen Type 1, Collagen type I alpha 1, Pro alpha 1(I) collagen, Type I procollagen, Collagen alpha-1(II) chain, Collagen alpha-1(II) chain, Chondrocalcin, collagen alpha-1(I) chain preproprotein, COL1.

Specifications of Mouse Collagen Type I ELISA kit

Product Information

Cat. No.

E03C0014

Product Name

Mouse Collagen Type I ELISA kit

Species

Mouse

Product Size

48 Tests / 96 Tests

Concentration

50-1000ng/mL

Sensitivity

1.0ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

50 ng/mL

1 vial

STANDARD C (0.5mL)

100 ng/mL

1 vial

STANDARD D (0.5mL)

250 ng/mL

1 vial

STANDARD E (0.5mL)

500 ng/mL

1 vial

STANDARD F (0.5mL)

1000 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

COL1 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-COL1 antibody and an COL1-HRP conjugate. The assay sample and buffer are incubated together with COL1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the COL1 concentration since COL1 from samples and COL1-HRP conjugate compete for the anti-COL1 antibody binding site. Since the number of sites is limited, as more sites are occupied by COL1 from the sample, fewer sites are left to bind COL1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The COL1 concentration in each sample is interpolated from this standard curve. 


Quality Control on Mouse Collagen Type I ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between COL1 and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Mouse Collagen Type I ELISA kit

Summary of the Assay Procedure for Mouse Collagen Type I ELISA kit

Citations of Mouse Collagen Type I ELISA kit

E03C0014 has been referenced in the below publications:

Enhanced osteogenic differentiation of osteoblasts on CaTiO3 nanotube film.

Ca substitution of Sr in Sr-doped TiO2 nanotube film on Ti surface for enhanced osteogenic activity.


Related Bluegene Biotech Products

Join us and become BlueGene's distributor, you will get a broad range of Life science research products and more than 12 years of experienced technical supports.