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BlueGene Biotech Mouse Interleukin 35 ELISA kit (E03I0389)

E03I0389 Mouse Interleukin 35 ELISA kit

The Mouse Interleukin 35 ELISA kit can be used to identify samples from the mouse species. Interleukin 35 can also be called IL 35.

Products

Specifications of Mouse Interleukin 35 ELISA kit

Product Information

Cat. No.

E03I0389

Product Name

Mouse Interleukin 35 ELISA kit

Species

Mouse

Product Size

48 Tests / 96 Tests

Concentration

50-1000pg/ml

Sensitivity

1.0pg/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/ml1 vial

STANDARD B (0.5mL)

50 pg/ml

1 vial

STANDARD C (0.5mL)

100 pg/ml

1 vial

STANDARD D (0.5mL)

250 pg/ml

1 vial

STANDARD E (0.5mL)

500 pg/ml

1 vial

STANDARD F (0.5mL)

1000 pg/ml

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

IL 35 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-IL 35 antibody and an IL 35-HRP conjugate. The assay sample and buffer are incubated together with IL 35-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IL 35 concentration since IL 35 from samples and IL 35-HRP conjugate compete for the anti-IL 35 antibody binding site. Since the number of sites is limited, as more sites are occupied by IL 35 from the sample, fewer sites are left to bind IL 35-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IL 35 concentration in each sample is interpolated from this standard curve. 


Quality Control on Mouse Interleukin 35 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between IL 35 and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Mouse Interleukin 35 ELISA kit

Summary of the Assay Procedure for Mouse Interleukin 35 ELISA kit

Citations of Mouse Interleukin 35 ELISA kit

E03I0389 has been referenced in the below publications:

IL-35 is elevated in clinical and experimental sepsis and mediates inflammation.

Interleukin-35 is upregulated in response to influenza virus infection and secondary bacterial pneumonia.



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