E03M0005 Mouse Melatonin ELISA kit
The Mouse Melatonin ELISA kit can be used to identify samples from the mouse species. Melatonin can also be called Melatonin, MLT.
Mouse Melatonin ELISA kit
48 Tests / 96 Tests
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
STANDARD A (0.5mL)
STANDARD B (0.5mL)
STANDARD C (0.5mL)
STANDARD D (0.5mL)
STANDARD E (0.5mL)
STANDARD F (0.5mL)
WASH SOLUTION (100 x)
Principle of the Assay
MLT ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for MLT. Standards or samples are then added to the microtiter plate wells and MLT if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of MLT present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for MLT are added to each well to “sandwich” the MLT immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain MLT and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MLT concentration in each sample is interpolated from this standard curve.
Coefficient of Variance
Intra Variation% ＜10%
Inter Variation% ＜12%
No significant cross-reactivity or interference between MLT and analogues was observed.
E03M0005 has been referenced in the below publications：
Melatonin inhibits the caspase-1/cytochrome c/caspase-3 cell death pathway, inhibits MT1 receptor loss and delays disease progression in a mouse model of amyotrophic lateral sclerosis.
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