E03T0504 Mouse Total Bile Acide ELISA kit
The Mouse Total Bile Acide ELISA kit can be used to identify samples from the mouse species. Total Bile Acide can also be called TBAM.
Specifications of Mouse Total Bile Acide ELISA kit
Product Information | |
Cat. No. | E03T0504 |
Product Name | Mouse Total Bile Acide ELISA kit |
Species | Mouse |
Product Size | 48 Tests / 96 Tests |
Concentration | 0.5-10umol/L |
Sensitivity | 0.1 umol/L |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 umol/L | 1 vial |
STANDARD B (0.5mL) | 0.5 umol/L | 1 vial |
STANDARD C (0.5mL) | 1.0 umol/L | 1 vial |
STANDARD D (0.5mL) | 2.5 umol/L | 1 vial |
STANDARD E (0.5mL) | 5.0 umol/L | 1 vial |
STANDARD F (0.5mL) | 10 umol/L | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
TBAM ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-TBAM antibody and an TBAM-HRP conjugate. The assay sample and buffer are incubated together with TBAM-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TBAM concentration since TBAM from samples and TBAM-HRP conjugate compete for the anti-TBAM antibody binding site. Since the number of sites is limited, as more sites are occupied by TBAM from the sample, fewer sites are left to bind TBAM-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TBAM concentration in each sample is interpolated from this standard curve. |
Quality Control on Mouse Total Bile Acide ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between TBAM and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Mouse Total Bile Acide ELISA kit
Citations of Mouse Total Bile Acide ELISA kit
E03T0504 has been referenced in the below publications：
Effects of medium-chain fatty acids on cholesterol metabolism and their mechanisms in mice.
