E07I0345 Porcine Interferon γ ELISA kit
The Porcine Interferon γ ELISA kit can be used to identify samples from the porcine species. Interferon γ can also be called IFNγ, IFG, IFI, IFN Gamma, IFNG.
Specifications of Porcine Interferon γ ELISA kit
Product Information | |
Cat. No. | E07I0345 |
Product Name | Porcine Interferon γ ELISA kit |
Species | Porcine |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000 pg/mL |
Sensitivity | 1.0 pg/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 50 pg/mL | 1 vial |
STANDARD C (0.5mL) | 100 pg/mL | 1 vial |
STANDARD D (0.5mL) | 250 pg/mL | 1 vial |
STANDARD E (0.5mL) | 500 pg/mL | 1 vial |
STANDARD F (0.5mL) | 1000 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
IFNγ ELISA kit uses an anti-IFNγ antibody and an IFNγ-HRP conjugate in a competitive enzyme immunoassay method. IFNγ-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-IFNγ antibody binding site between IFNγ from samples and IFNγ-HRP conjugate, the intensity of the color is inversely proportional to the concentration of IFNγ. Since the number of sites is limited, as more sites are occupied by IFNγ from the sample, fewer sites are left to bind IFNγ-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IFNγ concentration in each sample is interpolated from this standard curve. |
Quality Control on Porcine Interferon γ ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between IFNγ and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Porcine Interferon γ ELISA kit
Citations of Porcine Interferon γ ELISA kit
E07I0345 has been referenced in the below publications：
Effects of feeding an herbal preparation to sows on immunological performance of offspring.
Improved Cytotoxic T Lymphocyte Responses to Vaccination with Porcine Reproductive and Respiratory Syndrome Virus in 4-1BB Transgenic Pigs.
蜂胶乙醇提取物对断奶仔猪血液生理及免疫功能的影响。
