E02O0874 Rat 25 hydroxyvitamin D3 ELISA Kit
The Rat 25 hydroxyvitamin D3 ELISA Kit can be used to identify samples from the rat species. 25 hydroxyvitamin D3 can also be called 25-Hydroxyvitamin D3, 25-Hydroxycholecalciferol, Calcidiol, 25(OH)D3.
Specifications of Rat 25 hydroxyvitamin D3 ELISA Kit
Product Information | |
Cat. No. | E02O0874 |
Product Name | Rat 25 hydroxyvitamin D3 ELISA Kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 10-250 ng/mL |
Sensitivity | 1.0 ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 10 ng/mL | 1 vial |
STANDARD C (0.5mL) | 25 ng/mL | 1 vial |
STANDARD D (0.5mL) | 50 ng/mL | 1 vial |
STANDARD E (0.5mL) | 100 ng/mL | 1 vial |
STANDARD F (0.5mL) | 250 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
25(OH)D3 ELISA kit uses an anti-25(OH)D3 antibody and an 25(OH)D3-HRP conjugate in a competitive enzyme immunoassay method. 25(OH)D3-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-25(OH)D3 antibody binding site between 25(OH)D3 from samples and 25(OH)D3-HRP conjugate, the intensity of the color is inversely proportional to the concentration of 25(OH)D3. Since the number of sites is limited, as more sites are occupied by 25(OH)D3 from the sample, fewer sites are left to bind 25(OH)D3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The 25(OH)D3 concentration in each sample is interpolated from this standard curve. |
Quality Control on Rat 25 hydroxyvitamin D3 ELISA Kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between 25(OH)D3 and analogues was observed. | |
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Summary of the Assay Procedure for Rat 25 hydroxyvitamin D3 ELISA Kit
Citations of Rat 25 hydroxyvitamin D3 ELISA Kit
E02O0874 has been referenced in the below publications：
Cardiac metabolism, inflammation, and peroxisome proliferator-activated receptors modulated by 1, 25-dihydroxyvitamin D3 in diabetic rats.
