E02L0038 Rat Leptin ELISA kit
The Rat Leptin ELISA kit can be used to identify samples from the rat species. Leptin can also be called LEP, Leptin Murine Obesity Homolog, Leptin Precursor Obesity Factor, OB, Obese Protein, Obesity, Obesity factor, Obesity homolog mouse, Obesity Murine Homolog Leptin, OBS.
Product Information | |
Cat. No. | E02L0038 |
Product Name | Rat Leptin ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 2.5-50 ng/mL |
Sensitivity | 0.1 ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD C (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD D (0.5mL) | 10 ng/mL | 1 vial |
STANDARD E (0.5mL) | 25 ng/mL | 1 vial |
STANDARD F (0.5mL) | 50 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
Leptin ELISA kit uses an anti-Leptin antibody and an Leptin-HRP conjugate in a competitive enzyme immunoassay method. Leptin-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-Leptin antibody binding site between Leptin from samples and Leptin-HRP conjugate, the intensity of the color is inversely proportional to the concentration of Leptin. Since the number of sites is limited, as more sites are occupied by Leptin from the sample, fewer sites are left to bind Leptin-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Leptin concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between Leptin and analogues was observed. | |
E02L0038 has been referenced in the below publications：
Nonalcoholic fatty liver tissue of hepatitis TNFΑ, TGFΒ1, LEPTIN and MAPK expression and drug intervention.
The Effect of Acupuncture on Insulin-resistance in Obese Rats.
Qingrejiedu party of type 2 diabetic rats serum inflammation factors leptin IL resistance element-1 intervention study.
The effect of Leptin, Ghrelin with Obstractive Sleep Apnea Hypopnea Symptom.
The Study of Melatonin Regulating Lipid Metabolismin Male Rats.
Protective effects and mechanism of matrine on high-fructose-diet induced non-alcoholic fatty liver in rats.
Impact of Tangzhiping on Lipid Metabolism and Adipokine in the Ｒats of Insulin Ｒesistance.
Angiotensin‑converting enzyme‑2 improves diabetic nephropathy by targeting Smad7 for ubiquitin degradation.
Effect of melatonin on visceral fat deposition, lipid metabolism and hepatic lipo-metabolic gene expression in male rats.
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