E02H1417 Rat 3 Hydroxy 3 methylglutaryl CoA Elisa kit
The Rat 3 Hydroxy 3 methylglutaryl CoA Elisa kit can be used to identify samples from the rat species. 3 Hydroxy 3 methylglutaryl CoA can also be called 3-hydroxy-3-methylglutaryl CoA, 3-hydroxy-3-methylglutaryl coenzyme A, HMG-CoA.
Product Information | |
Cat. No. | E02H1417 |
Product Name | Rat 3 Hydroxy 3 methylglutaryl CoA Elisa kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 1.0-25 ng/mL |
Sensitivity | 0.1ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 1.0 ng/mL | 1 vial |
STANDARD C (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD D (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD E (0.5mL) | 10 ng/mL | 1 vial |
STANDARD F (0.5mL) | 25 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
HMG-CoA ELISA kit uses an anti-HMG-CoA antibody and an HMG-CoA-HRP conjugate in a competitive enzyme immunoassay method. HMG-CoA-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-HMG-CoA antibody binding site between HMG-CoA from samples and HMG-CoA-HRP conjugate, the intensity of the color is inversely proportional to the concentration of HMG-CoA. Since the number of sites is limited, as more sites are occupied by HMG-CoA from the sample, fewer sites are left to bind HMG-CoA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HMG-CoA concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between HMG-CoA and analogues was observed. | |
E02H1417 has been referenced in the below publications：
THE STUDY ON REGULATING EFFECTS AND MECHANISM OF LAMINARLA JAPONICA ON SERUM LIPID OF EXPERIMENTAL HYPERLIPENMIA.
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