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BlueGene Biotech Rat Chemokine Ligand 12 ELISA Kit

E02S0067 Rat Chemokine  Ligand 12  ELISA Kit

The Rat Chemokine  Ligand 12  ELISA Kit can be used to identify samples from the rat species. Chemokine  Ligand 12 can also be called SDF1 alpha, C-X-C motif chemokine 12, Chemokine (C X C motif) ligand 12, CXCL12, hIRH, hSDF-1, Intercrine reduced in hepatomas, IRH, PBSF, PBSF, Pre B cell growth stimulating factor, Pre-B cell growth-stimulating factor, SCYB12, SDF 1 alpha, SDF-1, SDF 1 beta, SDF 1b, SDF-1, SDF-1-alpha(3-67), SDF1.

Specifications of Rat Chemokine Ligand 12 ELISA Kit

Product Information

Cat. No.

E02S0067

Product Name

Rat Chemokine  Ligand 12  ELISA Kit

Species

Rat

Product Size

48 Tests / 96 Tests

Concentration

250-5000 pg/mL

Sensitivity

1.0 pg/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

250 pg/mL

1 vial

STANDARD C (0.5mL)

500 pg/mL

1 vial

STANDARD D (0.5mL)

1000 pg/mL

1 vial

STANDARD E (0.5mL)

2500 pg/mL

1 vial

STANDARD F (0.5mL)

5000 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

CXCL12 ELISA kit uses an anti-CXCL12 antibody and an CXCL12-HRP conjugate in a competitive enzyme immunoassay method. CXCL12-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-CXCL12 antibody binding site between CXCL12 from samples and CXCL12-HRP conjugate, the intensity of the color is inversely proportional to the concentration of CXCL12. Since the number of sites is limited, as more sites are occupied by CXCL12 from the sample, fewer sites are left to bind CXCL12-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CXCL12 concentration in each sample is interpolated from this standard curve.


Quality Control on Rat Chemokine Ligand 12 ELISA Kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between CXCL12 and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rat Chemokine Ligand 12 ELISA Kit

Summary of the Assay Procedure for Rat Chemokine  Ligand 12  ELISA Kit

Citations of Rat Chemokine Ligand 12 ELISA Kit

E02S0067 has been referenced in the below publications:

Anti-Thymocyte Globulin Induces Neoangiogenesis and Preserves Cardiac Function after Experimental Myocardial Infarction.


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