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  • bovine serum albumin elisa 1

BlueGene Biotech Rat Cholecystokinin ELISA kit

E02C0376 Rat Cholecystokinin ELISA kit

The Rat Cholecystokinin ELISA kit can be used to identify samples from the rat species. Cholecystokinin can also be called CCK, CCK-PZ.

Specifications of Rat Cholecystokinin ELISA kit

Product Information

Cat. No.

E02C0376

Product Name

Rat Cholecystokinin ELISA kit

Species

Rat

Product Size

48 Tests / 96 Tests

Concentration

50-1000 pg/ml

Sensitivity

1.0 pg/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 pg/mL

1 vial

STANDARD B (0.5mL)

50 pg/mL

1 vial

STANDARD C (0.5mL)

100 pg/mL

1 vial

STANDARD D (0.5mL)

250 pg/mL

1 vial

STANDARD E (0.5mL)

500 pg/mL

1 vial

STANDARD F (0.5mL)

1000 pg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

CCK ELISA kit uses an anti-CCK antibody and an CCK-HRP conjugate in a competitive enzyme immunoassay method. CCK-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-CCK antibody binding site between CCK from samples and CCK-HRP conjugate, the intensity of the color is inversely proportional to the concentration of CCK. Since the number of sites is limited, as more sites are occupied by CCK from the sample, fewer sites are left to bind CCK-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CCK concentration in each sample is interpolated from this standard curve.


Quality Control on Rat Cholecystokinin ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between CCK and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rat Cholecystokinin ELISA kit

Summary of the Assay Procedure for Rat Cholecystokinin ELISA kit

Citations of Rat Cholecystokinin ELISA kit

E02C0376 has been referenced in the below publications:

电针对脾虚消化不良大鼠胃肠激素的影响。

Effect of Liraglutide on improving the glucose metabolism by inhibiting toll-like recpter 4/ toll-like recpter 4 signaling pathway in type 2 diabetes rats.

Effect of Zheng-Qing Feng-Tong-Ning on Osteoporotic Vertebral Fracture in Rats.


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