E02C0376 Rat Cholecystokinin ELISA kit
The Rat Cholecystokinin ELISA kit can be used to identify samples from the rat species. Cholecystokinin can also be called CCK, CCK-PZ.
Specifications of Rat Cholecystokinin ELISA kit
Product Information | |
Cat. No. | E02C0376 |
Product Name | Rat Cholecystokinin ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 50-1000 pg/ml |
Sensitivity | 1.0 pg/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 pg/mL | 1 vial |
STANDARD B (0.5mL) | 50 pg/mL | 1 vial |
STANDARD C (0.5mL) | 100 pg/mL | 1 vial |
STANDARD D (0.5mL) | 250 pg/mL | 1 vial |
STANDARD E (0.5mL) | 500 pg/mL | 1 vial |
STANDARD F (0.5mL) | 1000 pg/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
CCK ELISA kit uses an anti-CCK antibody and an CCK-HRP conjugate in a competitive enzyme immunoassay method. CCK-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-CCK antibody binding site between CCK from samples and CCK-HRP conjugate, the intensity of the color is inversely proportional to the concentration of CCK. Since the number of sites is limited, as more sites are occupied by CCK from the sample, fewer sites are left to bind CCK-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CCK concentration in each sample is interpolated from this standard curve. |
Quality Control on Rat Cholecystokinin ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between CCK and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Rat Cholecystokinin ELISA kit
Citations of Rat Cholecystokinin ELISA kit
E02C0376 has been referenced in the below publications：
电针对脾虚消化不良大鼠胃肠激素的影响。
Effect of Liraglutide on improving the glucose metabolism by inhibiting toll-like recpter 4/ toll-like recpter 4 signaling pathway in type 2 diabetes rats.
Effect of Zheng-Qing Feng-Tong-Ning on Osteoporotic Vertebral Fracture in Rats.
