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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Rat Cluster of Differentiation 8 ELISA kit (E02C0007)

E02C0007 Rat Cluster of Differentiation 8 ELISA kit

The Rat Cluster of Differentiation 8 ELISA kit can be used to identify samples from the rat species. Cluster of Differentiation 8 can also be called P32, CD8-A, Leu2, MAL, T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2.

Products

Specifications of Rat Cluster of Differentiation 8 ELISA kit

Product Information

Cat. No.

E02C0007

Product Name

Rat Cluster of Differentiation 8 ELISA kit

Species

Rat

Product Size

48 Tests / 96 Tests

Concentration

1.0-25 ng/mL

Sensitivity

0.1ng/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

1.0 ng/mL

1 vial

STANDARD C (0.5mL)

2.5 ng/mL

1 vial

STANDARD D (0.5mL)

5.0 ng/mL

1 vial

STANDARD E (0.5mL)

10 ng/mL

1 vial

STANDARD F (0.5mL)

25 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

CD8 ELISA kit uses an anti-CD8 antibody and an CD8-HRP conjugate in a competitive enzyme immunoassay method. CD8-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-CD8 antibody binding site between CD8 from samples and CD8-HRP conjugate, the intensity of the color is inversely proportional to the concentration of CD8. Since the number of sites is limited, as more sites are occupied by CD8 from the sample, fewer sites are left to bind CD8-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CD8 concentration in each sample is interpolated from this standard curve.


Quality Control on Rat Cluster of Differentiation 8 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between CD8 and analogues was observed.


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Summary of the Assay Procedure for Rat Cluster of Differentiation 8 ELISA kit

Summary of the Assay Procedure for Rat Cluster of Differentiation 8 ELISA kit

Citations of Rat Cluster of Differentiation 8 ELISA kit

E02C0007 has been referenced in the below publications:

Effects of Benzene Exposure on Cellular lmmune and Micronucleus Generation in rats Weightlessness Simulated by Tail-suspension.

Effects of Formaldehyde Exposure on Cellular Immune and Micronucleus Generation in Rats Under Simulated Weightlessness Environment.

Effects of Bushen Yiqi Huayu Prescription on Immune Function of Aging Rats.


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