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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Rat Alpha Amylase ELISA kit

E02A0010 Rat Alpha Amylase ELISA kit

Rat Alpha Amylase ELISA kit is suitable for the detection of samples from Rat species. Alpha Amylase can also be called glycogenase, α amylase, endoamylase, Taka-amylase A, 1,4-α-D-glucan glucanohydrolase, AMY.

Specifications of Rat Alpha Amylase ELISA kit

Product Information

Cat. No.

E02A0010

Product Name

Rat Alpha Amylase ELISA kit

Species

Rat

Product Size

48 Tests / 96 Tests

Concentration

10-250ng/ml

Sensitivity

1.0 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

10 ng/mL

1 vial

STANDARD C (0.5mL)

25 ng/mL

1 vial

STANDARD D (0.5mL)

50 ng/mL

1 vial

STANDARD E (0.5mL)

100 ng/mL

1 vial

STANDARD F (0.5mL)

250 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

AMY ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-AMY antibody and an AMY-HRP conjugate. The assay sample and buffer are incubated together with AMY-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AMY concentration since AMY from samples and AMY-HRP conjugate compete for the anti-AMY antibody binding site. Since the number of sites is limited, as more sites are occupied by AMY from the sample, fewer sites are left to bind AMY-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AMY concentration in each sample is interpolated from this standard curve.


Quality Control on Rat Alpha Amylase ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

94-106%

Linearity

Diluent Ratio

Range %

1:2

95-103

1:4

93-108

1:8

90-112

Specificity/Cross-reactivity

No significant cross-reactivity or interference between AMY and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rat Alpha Amylase ELISA kit

Summary of the Assay Procedure for Rat Alpha Amylase ELISA kit

Citations of Rat Alpha Amylase ELISA kit

E02A0010 has been referenced in the below publications:

The protective effect and mechanism of the alanyl-glutamine dipeptide in the treatment of severe acute pancreatitis.

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