E02C0006 Rat Corticosterone ELISA kit
The Rat Corticosterone ELISA kit can be used to identify samples from the rat species. Corticosterone can also be called CORT, Corticosrone.
Rat Corticosterone ELISA kit
48 Tests / 96 Tests
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
STANDARD A (0.5mL)
STANDARD B (0.5mL)
STANDARD C (0.5mL)
STANDARD D (0.5mL)
STANDARD E (0.5mL)
STANDARD F (0.5mL)
WASH SOLUTION (100 x)
Principle of the Assay
CORT ELISA kit uses an anti-CORT antibody and an CORT-HRP conjugate in a competitive enzyme immunoassay method. CORT-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-CORT antibody binding site between CORT from samples and CORT-HRP conjugate, the intensity of the color is inversely proportional to the concentration of CORT. Since the number of sites is limited, as more sites are occupied by CORT from the sample, fewer sites are left to bind CORT-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CORT concentration in each sample is interpolated from this standard curve.
Coefficient of Variance
Intra Variation% ＜10%
Inter Variation% ＜12%
No significant cross-reactivity or interference between CORT and analogues was observed.
E02C0006 has been referenced in the below publications：
Effects of chronic noise on glucose metabolism and gut microbiota-host inflammatory homeostasis in rats.
Experimental Study of Herba Epimedii and Fructus Ligustri Lucid on the Glucocorticoid-induced Osteoporosis Rats based on regulation of TGF-beta 1/Smads signaling pathway.
Effects of the combination of Epimedii Folium and Fructus Ligustri Lucidi on glucocorticoid receptor in glucocorticoid-induced osteoporosis rats.
Transcutaneous Auricular Vagus Nerve Stimulation at 20 Hz Improves Depression-Like Behaviors and Down-Regulates the Hyperactivity of HPA Axis in Chronic Unpredictable Mild Stress Model Rats.
Effects of chronic noise on mRNA and protein expression of CRF family molecules and its relationship with p-tau in the rat prefrontal cortex.
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