E02C0027 Rat cyclic Adenosine Monophosphate ELISA kit
The Rat cyclic Adenosine Monophosphate ELISA kit can be used to identify samples from the rat species. cyclic Adenosine Monophosphate can also be called cAMP, cyclic AMP, 3',5'-cyclic adenosine monophosphate.
Product Information | |
Cat. No. | E02C0027 |
Product Name | Rat cyclic Adenosine Monophosphate ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 5.0-100 ng/ml |
Sensitivity | 1.0 ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD C (0.5mL) | 10 ng/mL | 1 vial |
STANDARD D (0.5mL) | 25 ng/mL | 1 vial |
STANDARD E (0.5mL) | 50 ng/mL | 1 vial |
STANDARD F (0.5mL) | 100 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
cAMP ELISA kit uses an anti-cAMP antibody and an cAMP-HRP conjugate in a competitive enzyme immunoassay method. cAMP-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-cAMP antibody binding site between cAMP from samples and cAMP-HRP conjugate, the intensity of the color is inversely proportional to the concentration of cAMP. Since the number of sites is limited, as more sites are occupied by cAMP from the sample, fewer sites are left to bind cAMP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The cAMP concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between cAMP and analogues was observed. | |
E02C0027 has been referenced in the below publications：
Effect of Regulating Liver and Invigorating Spleen Decoction on the function of antral mucosa and smooth muscle in rats with functional dyspepsia ( syndrome of liver-qi stagnation and spleen-qi deficidency ).
Serum heparanase levels: A protective marker against digital ulcers in patients with systemic sclerosis.
Relaxation of Danhong Injection in vascular smooth muscle cells in co-culture system and study on its mechanism.
Study on the protective effects of Qishen Yiqi pill on myocardial ischemia reperfusion injury in rats.
Edaravone improves intermittent hypoxia-induced cognitive impairment and hippocampal damage in rats.
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