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  • bovine serum albumin elisa 1

BlueGene Biotech Rat cyclic Adenosine Monophosphate ELISA kit (E02C0027)

E02C0027 Rat cyclic Adenosine Monophosphate ELISA kit

The Rat cyclic Adenosine Monophosphate ELISA kit can be used to identify samples from the rat species. cyclic Adenosine Monophosphate can also be called cAMP, cyclic AMP, 3',5'-cyclic adenosine monophosphate.

Products

Specifications of Rat cyclic Adenosine Monophosphate ELISA kit

Product Information

Cat. No.

E02C0027

Product Name

Rat cyclic Adenosine Monophosphate ELISA kit

Species

Rat

Product Size

48 Tests / 96 Tests

Concentration

5.0-100 ng/ml

Sensitivity

1.0 ng/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

5.0 ng/mL

1 vial

STANDARD C (0.5mL)

10 ng/mL

1 vial

STANDARD D (0.5mL)

25 ng/mL

1 vial

STANDARD E (0.5mL)

50 ng/mL

1 vial

STANDARD F (0.5mL)

100 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

cAMP ELISA kit uses an anti-cAMP antibody and an cAMP-HRP conjugate in a competitive enzyme immunoassay method. cAMP-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-cAMP antibody binding site between cAMP from samples and cAMP-HRP conjugate, the intensity of the color is inversely proportional to the concentration of cAMP. Since the number of sites is limited, as more sites are occupied by cAMP from the sample, fewer sites are left to bind cAMP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The cAMP concentration in each sample is interpolated from this standard curve.


Quality Control on Rat cyclic Adenosine Monophosphate ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between cAMP and analogues was observed.


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Summary of the Assay Procedure for Rat cyclic Adenosine Monophosphate ELISA kit

Summary of the Assay Procedure for Rat cyclic Adenosine Monophosphate ELISA kit

Citations of Rat cyclic Adenosine Monophosphate ELISA kit

E02C0027 has been referenced in the below publications:

Effect of Regulating Liver and Invigorating Spleen Decoction on the function of antral mucosa and smooth muscle in rats with functional dyspepsia ( syndrome of liver-qi stagnation and spleen-qi deficidency ).

Serum heparanase levels: A protective marker against digital ulcers in patients with systemic sclerosis.

Relaxation of Danhong Injection in vascular smooth muscle cells in co-culture system and study on its mechanism.

Study on the protective effects of Qishen Yiqi pill on myocardial ischemia reperfusion injury in rats.

Edaravone improves intermittent hypoxia-induced cognitive impairment and hippocampal damage in rats.


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