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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Rat D Dimer ELISA kit

E02D0224 Rat D Dimer ELISA kit

The Rat D Dimer ELISA kit can be used to identify samples from the rat species. D Dimer can also be called D2D, D 2 Dimer, D-Dimer.

Specifications of Rat D Dimer ELISA kit

Product Information

Cat. No.

E02D0224

Product Name

Rat D Dimer ELISA kit

Species

Rat

Product Size

48 Tests / 96 Tests

Concentration

250-5000 ng/ml

Sensitivity

1.0 ng/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

250 ng/mL

1 vial

STANDARD C (0.5mL)

500 ng/mL

1 vial

STANDARD D (0.5mL)

1000 ng/mL

1 vial

STANDARD E (0.5mL)

2500 ng/mL

1 vial

STANDARD F (0.5mL)

5000 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

D2D ELISA kit uses an anti-D2D antibody and an D2D-HRP conjugate in a competitive enzyme immunoassay method. D2D-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-D2D antibody binding site between D2D from samples and D2D-HRP conjugate, the intensity of the color is inversely proportional to the concentration of D2D. Since the number of sites is limited, as more sites are occupied by D2D from the sample, fewer sites are left to bind D2D-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The D2D concentration in each sample is interpolated from this standard curve.


Quality Control on Rat D Dimer ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between D2D and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rat D Dimer ELISA kit

Summary of the Assay Procedure for Rat D Dimer ELISA kit

Citations of Rat D Dimer ELISA kit

E02D0224 has been referenced in the below publications:

化瘀解毒法对急性肺血栓栓塞症兔D-二聚体、内皮素-1、B型脑利钠肽表达影响。


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