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BlueGene Biotech Rat Endothelial Nitric Oxide Synthase ELISA kit (E02N0024)

E02N0024 Rat Endothelial Nitric Oxide Synthase ELISA kit

The Rat Endothelial Nitric Oxide Synthase ELISA kitcan be used to identify samples from the rat species. Endothelial Nitric Oxide Synthase can also be called NOS-3, cNOS, Constitutive NOS, ecNOS, Endothelial nitric oxidase synthase, Endothelial nitric oxide synthase 3, Endothelial NOS, Nitric oxide synthase 3 (endothelial cell), Nitric oxide synthase 3, Nitric oxide synthase 3 endothelial cell, Nitric oxide synthase endothelial, nitric oxide synthase, endothelial, NOS 3, NOS III, NOS type III.

Products

Specifications of Rat Endothelial Nitric Oxide Synthase ELISA kit

Product Information

Cat. No.

E02N0024

Product Name

Rat Endothelial Nitric Oxide Synthase ELISA kit

Species

Rat

Product Size

48 Tests / 96 Tests

Concentration

2.5-50 ng/mL

Sensitivity

0.1 ng/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

2.5 ng/mL

1 vial

STANDARD C (0.5mL)

5.0 ng/mL

1 vial

STANDARD D (0.5mL)

10 ng/mL

1 vial

STANDARD E (0.5mL)

25 ng/mL

1 vial

STANDARD F (0.5mL)

50 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

eNOS ELISA kit uses an anti-eNOS antibody and an eNOS-HRP conjugate in a competitive enzyme immunoassay method. eNOS-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-eNOS antibody binding site between eNOS from samples and eNOS-HRP conjugate, the intensity of the color is inversely proportional to the concentration of eNOS. Since the number of sites is limited, as more sites are occupied by eNOS from the sample, fewer sites are left to bind eNOS-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The eNOS concentration in each sample is interpolated from this standard curve.


Quality Control on Rat Endothelial Nitric Oxide Synthase ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between eNOS and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rat Endothelial Nitric Oxide Synthase ELISA kit

Summary of the Assay Procedure for Rat Endothelial Nitric Oxide Synthase ELISA kit

Citations of Rat Endothelial Nitric Oxide Synthase ELISA kit

E02N0024 has been referenced in the below publications:

Damage effects of high iodine on endothelial cells from the rat aorta segments.

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