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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Rat Fatty Acid Binding Protein 1, Liver (FABP1) ELISA Kit

E02L0300 Rat Fatty Acid Binding Protein 1, Liver ELISA Kit

The Rat Fatty Acid Binding Protein 1, Liver ELISA Kit can be used to identify samples from the rat species. Fatty Acid Binding Protein 1, Liver can also be called LFABP, FABP1, FABPL, L-FABP, fatty acid binding protein 1.

Specifications of Rat Fatty Acid Binding Protein 1, Liver ELISA Kit

Product Information

Cat. No.

E02L0300

Product Name

Rat Fatty Acid Binding Protein 1, Liver ELISA Kit

Species

Rat

Product Size

48 Tests / 96 Tests

Concentration

2.5-50 ng/mL

Sensitivity

0.1 ng/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

2.5 ng/mL

1 vial

STANDARD C (0.5mL)

5.0 ng/mL

1 vial

STANDARD D (0.5mL)

10 ng/mL

1 vial

STANDARD E (0.5mL)

25 ng/mL

1 vial

STANDARD F (0.5mL)

50 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

FABP1 ELISA kit uses an anti-FABP1 antibody and an FABP1-HRP conjugate in a competitive enzyme immunoassay method. FABP1-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-FABP1 antibody binding site between FABP1 from samples and FABP1-HRP conjugate, the intensity of the color is inversely proportional to the concentration of FABP1. Since the number of sites is limited, as more sites are occupied by FABP1 from the sample, fewer sites are left to bind FABP1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FABP1 concentration in each sample is interpolated from this standard curve.


Quality Control on Rat Fatty Acid Binding Protein 1, Liver ELISA Kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between FABP1 and analogues was observed.


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Summary of the Assay Procedure for Rat Fatty Acid Binding Protein 1, Liver ELISA Kit

Summary of the Assay Procedure for Rat Fatty Acid Binding Protein 1, Liver ELISA Kit

Citations of Rat Fatty Acid Binding Protein 1, Liver ELISA Kit

E02L0300 has been referenced in the below publications:

Value of serum liver fatty acid-binding protein in monitoring of hepatic function after the ischemiareperfusion injury.

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