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  • bovine serum albumin elisa 1

BlueGene Biotech Rat Hypoxia Inducible Factor 1, Alpha ELISA kit

E02H0061 Rat Hypoxia Inducible Factor 1, Alpha ELISA kit

The Rat Hypoxia Inducible Factor 1, Alpha ELISA kit can be used to identify samples from the rat species. Hypoxia Inducible Factor 1, Alpha can also be called HIF1A; HIF-1-alpha; HIF-1A; HIF-1alpha; HIF1; HIF1-ALPHA; MOP1; PASD8; bHLHe78; hypoxia inducible factor 1 alpha subunit; hypoxia inducible factor 1 subunit alpha; HIF-1α.

Specifications of Rat Hypoxia Inducible Factor 1, Alpha ELISA kit

Product Information

Cat. No.

E02H0061

Product Name

Rat Hypoxia Inducible Factor 1, Alpha ELISA kit

Species

Rat

Product Size

48 Tests / 96 Tests

Concentration

0.5-10 ng/ml

Sensitivity

0.1 ng/ml

Principal

Competitive ELISA

Sample Volume

50 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

10 mL

1 vial

STANDARD A (0.5mL)

0 ng/ml

1 vial

STANDARD B (0.5mL)

0.5 ng/ml

1 vial

STANDARD C (0.5mL)

1.0 ng/ml

1 vial

STANDARD D (0.5mL)

2.5 ng/ml

1 vial

STANDARD E (0.5mL)

5.0 ng/ml

1 vial

STANDARD F (0.5mL)

10 ng/ml

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

HIF 1Alpha ELISA kit uses an anti-HIF 1Alpha antibody and an HIF 1Alpha-HRP conjugate in a competitive enzyme immunoassay method. HIF 1Alpha-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-HIF 1Alpha antibody binding site between HIF 1Alpha from samples and HIF 1Alpha-HRP conjugate, the intensity of the color is inversely proportional to the concentration of HIF 1Alpha. Since the number of sites is limited, as more sites are occupied by HIF 1Alpha from the sample, fewer sites are left to bind HIF 1Alpha-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HIF 1Alpha concentration in each sample is interpolated from this standard curve.


Quality Control on Rat Hypoxia Inducible Factor 1, Alpha ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between HIF 1Alpha and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rat Hypoxia Inducible Factor 1, Alpha ELISA kit

Summary of the Assay Procedure for Rat Hypoxia Inducible Factor 1, Alpha ELISA kit

Citations of Rat Hypoxia Inducible Factor 1, Alpha ELISA kit

E02H0061 has been referenced in the below publications:

Antitumor effect of a selective COX-2 inhibitor, celecoxib,may be attributed to angiogenesis inhibition through modulating the PTEN/PI3K/Akt/HIF-1 pathway in an H22 murine hepatocarcinoma model.

Role of plateau hypoxia hypothermia complex damage to the vascular endothelial cells and the protective effect.

麻黄、五味子配伍地塞米松对肺纤维化大鼠代谢差异性研究。

Effects of alcohol extract from root of wintersweet on expression of HIF-1α,VEGF and MMP-3 in adjuvant arthritis rats.

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