E02H0061 Rat Hypoxia Inducible Factor 1, Alpha ELISA kit
The Rat Hypoxia Inducible Factor 1, Alpha ELISA kit can be used to identify samples from the rat species. Hypoxia Inducible Factor 1, Alpha can also be called HIF1A; HIF-1-alpha; HIF-1A; HIF-1alpha; HIF1; HIF1-ALPHA; MOP1; PASD8; bHLHe78; hypoxia inducible factor 1 alpha subunit; hypoxia inducible factor 1 subunit alpha; HIF-1α.
Product Information | |
Cat. No. | E02H0061 |
Product Name | Rat Hypoxia Inducible Factor 1, Alpha ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 0.5-10 ng/ml |
Sensitivity | 0.1 ng/ml |
Principal | Competitive ELISA |
Sample Volume | 50 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 10 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/ml | 1 vial |
STANDARD B (0.5mL) | 0.5 ng/ml | 1 vial |
STANDARD C (0.5mL) | 1.0 ng/ml | 1 vial |
STANDARD D (0.5mL) | 2.5 ng/ml | 1 vial |
STANDARD E (0.5mL) | 5.0 ng/ml | 1 vial |
STANDARD F (0.5mL) | 10 ng/ml | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
HIF 1Alpha ELISA kit uses an anti-HIF 1Alpha antibody and an HIF 1Alpha-HRP conjugate in a competitive enzyme immunoassay method. HIF 1Alpha-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-HIF 1Alpha antibody binding site between HIF 1Alpha from samples and HIF 1Alpha-HRP conjugate, the intensity of the color is inversely proportional to the concentration of HIF 1Alpha. Since the number of sites is limited, as more sites are occupied by HIF 1Alpha from the sample, fewer sites are left to bind HIF 1Alpha-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HIF 1Alpha concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between HIF 1Alpha and analogues was observed. | |
E02H0061 has been referenced in the below publications：
Antitumor effect of a selective COX-2 inhibitor, celecoxib,may be attributed to angiogenesis inhibition through modulating the PTEN/PI3K/Akt/HIF-1 pathway in an H22 murine hepatocarcinoma model.
Role of plateau hypoxia hypothermia complex damage to the vascular endothelial cells and the protective effect.
麻黄、五味子配伍地塞米松对肺纤维化大鼠代谢差异性研究。
Effects of alcohol extract from root of wintersweet on expression of HIF-1α,VEGF and MMP-3 in adjuvant arthritis rats.
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