E02M0030 Rat Metallothionein ELISA kit
The Rat Metallothionein ELISA kit can be used to identify samples from the rat species. Metallothionein can also be called CES 1; MT 2;MT 2A; MT IA; MT II; MT1, MT.
Rat Metallothionein ELISA kit
48 Tests / 96 Tests
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
STANDARD A (0.5mL)
STANDARD B (0.5mL)
STANDARD C (0.5mL)
STANDARD D (0.5mL)
STANDARD E (0.5mL)
STANDARD F (0.5mL)
WASH SOLUTION (100 x)
Principle of the Assay
MT ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for MT. Standards or samples are then added to the microtiter plate wells and MT if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of MT present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for MT are added to each well to “sandwich” the MT immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain MT and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MT concentration in each sample is interpolated from this standard curve.
Coefficient of Variance
Intra Variation% ＜10%
Inter Variation% ＜12%
No significant cross-reactivity or interference between MT and analogues was observed.
E02M0030 has been referenced in the below publications：
Effect of Melatonin Treatment during Pregnancy on Growth of Female Offspring in Rat.
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