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  • bovine serum albumin elisa 1

BlueGene Biotech Rat Nephrin ELISA kit (E02N0079)

E02N0079 Rat Nephrin ELISA kit

The Rat Nephrin ELISA kit can be used to identify samples from the rat species. Nephrin can also be called NPHS1; CNF; Nephrosis 1; Congenital Finnish Type; Renal glomerulus-specific cell adhesion receptor.

Products

Specifications of Rat Nephrin ELISA kit

Product Information

Cat. No.

E02N0079

Product Name

Rat Nephrin ELISA kit

Species

Rat

Product Size

48 Tests / 96 Tests

Concentration

1.0-25 ng/mL

Sensitivity

0.1ng/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

1.0 ng/mL

1 vial

STANDARD C (0.5mL)

2.5 ng/mL

1 vial

STANDARD D (0.5mL)

5.0 ng/mL

1 vial

STANDARD E (0.5mL)

10 ng/mL

1 vial

STANDARD F (0.5mL)

25ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

Nephrin ELISA kit uses an anti-Nephrin antibody and an Nephrin-HRP conjugate in a competitive enzyme immunoassay method. Nephrin-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-Nephrin antibody binding site between Nephrin from samples and Nephrin-HRP conjugate, the intensity of the color is inversely proportional to the concentration of Nephrin. Since the number of sites is limited, as more sites are occupied by Nephrin from the sample, fewer sites are left to bind Nephrin-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Nephrin concentration in each sample is interpolated from this standard curve.


Quality Control on Rat Nephrin ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between Nephrin and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rat Nephrin ELISA kit

Summary of the Assay Procedure for Rat Nephrin ELISA kit

Citations of Rat Nephrin ELISA kit

E02N0079 has been referenced in the below publications:

Relationship  between urinary nephrin and urinary albumin chang- es in diabetic rats and effects of Yiqiyangyinhuayutongluo Recipe.

effects of chinese materla medica of qi beneflting,yin nourishing,stasis removing,and collaterals dredging and their dissermbled recipes on nephrin of diabetes meliltus model rats.

Effects of yiqi-yangyin-huoxue-tongluo recipe on podocyte of diabetic nephropathy rats.


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