E02S0299 Rat Stromelysin-2 ELISA
The Rat Stromelysin-2 ELISA can be used to identify samples from the rat species. Stromelysin-2 can also be called MMP-10, MMP10, SL-2, STMY2, Stromelysin-2, Transin-2.
Specifications of Rat Stromelysin-2 ELISA
Product Information | |
Cat. No. | E02S0299 |
Product Name | Rat Stromelysin-2 ELISA |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 0.5-10 ng/mL |
Sensitivity | 0.1 ng/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 0.5 ng/mL | 1 vial |
STANDARD C (0.5mL) | 1.0 ng/mL | 1 vial |
STANDARD D (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD E (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD F (0.5mL) | 10 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
ST2 ELISA kit uses an anti-ST2 antibody and an ST2-HRP conjugate in a competitive enzyme immunoassay method. ST2-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-ST2 antibody binding site between ST2 from samples and ST2-HRP conjugate, the intensity of the color is inversely proportional to the concentration of ST2. Since the number of sites is limited, as more sites are occupied by ST2 from the sample, fewer sites are left to bind ST2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ST2 concentration in each sample is interpolated from this standard curve. |
Quality Control on Rat Stromelysin-2 ELISA
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between ST2 and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Rat Stromelysin-2 ELISA
Citations of Rat Stromelysin-2 ELISA
E02S0299 has been referenced in the below publications：
Effects of exercise traning on ventricular remodeling following myocardial infarction in rats.
