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BlueGene Biotech Rat Nicotinamide adenine dinucleotide phosphate ELISA kit (E02N0067)

E02N0067 Rat Nicotinamide adenine dinucleotide phosphate ELISA kit

The Rat Nicotinamide adenine dinucleotide phosphate ELISA kit can be used to identify samples from the rat species. Nicotinamide adenine dinucleotide phosphate can also be called nicotinamide adenine dinucleotide phosphate, NADP, NADPH, TPNH, Triphosphopyridine nucleotide, reduced form,   β-NADPH.

Products

Specifications of Rat Nicotinamide adenine dinucleotide phosphate ELISA kit

Product Information

Cat. No.

E02N0067

Product Name

Rat Nicotinamide adenine dinucleotide phosphate ELISA kit

Species

Rat

Product Size

48 Tests / 96 Tests

Concentration

5.0-100 ng/ml

Sensitivity

1.0 ng/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

5.0 ng/mL

1 vial

STANDARD C (0.5mL)

10 ng/mL

1 vial

STANDARD D (0.5mL)

25 ng/mL

1 vial

STANDARD E (0.5mL)

50 ng/mL

1 vial

STANDARD F (0.5mL)

100 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

NADPH ELISA kit uses an anti-NADPH antibody and an NADPH-HRP conjugate in a competitive enzyme immunoassay method. NADPH-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-NADPH antibody binding site between NADPH from samples and NADPH-HRP conjugate, the intensity of the color is inversely proportional to the concentration of NADPH. Since the number of sites is limited, as more sites are occupied by NADPH from the sample, fewer sites are left to bind NADPH-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NADPH concentration in each sample is interpolated from this standard curve.


Quality Control on Rat Nicotinamide adenine dinucleotide phosphate ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between NADPH and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rat Nicotinamide adenine dinucleotide phosphate ELISA kit

Summary of the Assay Procedure for Rat Nicotinamide adenine dinucleotide phosphate ELISA kit

Citations of Rat Nicotinamide adenine dinucleotide phosphate ELISA kit

E02N0067 has been referenced in the below publications:

The Role of Resolvin D1 on acute reflux esophagitis with DHA intervention in Rats.

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