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BlueGene Biotech Rat Pigment Epithelium Derived Factor ELISA kit

E02P0084 Rat Pigment Epithelium Derived Factor ELISA kit

The Rat Pigment Epithelium Derived Factor ELISA kit can be used to identify samples from the rat species. Pigment Epithelium Derived Factor can also be called SERPINF1, EPC1, PIG35, SDF3, Serpin F1, Cell proliferation-inducing gene 35, Serpin Peptidase Inhibitor,Clade F Member 1(Alpha-2 Antiplasmin), Stromal Cell Derived Factor 3.

Specifications of Rat Pigment Epithelium Derived Factor ELISA kit

Product Information

Cat. No.

E02P0084

Product Name

Rat Pigment Epithelium Derived Factor ELISA kit

Species

Rat

Product Size

48 Tests / 96 Tests

Concentration

5.0-100 ng/ml

Sensitivity

1.0 ng/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

5.0 ng/mL

1 vial

STANDARD C (0.5mL)

10 ng/mL

1 vial

STANDARD D (0.5mL)

25 ng/mL

1 vial

STANDARD E (0.5mL)

50 ng/mL

1 vial

STANDARD F (0.5mL)

100 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

PEDF ELISA kit uses an anti-PEDF antibody and an PEDF-HRP conjugate in a competitive enzyme immunoassay method. PEDF-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-PEDF antibody binding site between PEDF from samples and PEDF-HRP conjugate, the intensity of the color is inversely proportional to the concentration of PEDF. Since the number of sites is limited, as more sites are occupied by PEDF from the sample, fewer sites are left to bind PEDF-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PEDF concentration in each sample is interpolated from this standard curve.


Quality Control on Rat Pigment Epithelium Derived Factor ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between PEDF and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rat Pigment Epithelium Derived Factor ELISA kit

Summary of the Assay Procedure for Rat Pigment Epithelium Derived Factor ELISA kit

Citations of Rat Pigment Epithelium Derived Factor ELISA kit

E02P0084 has been referenced in the below publications:

麻黄、五味子配伍地塞米松对肺纤维化大鼠代谢差异性研究。


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