E02T0143 Rat Total Bilirubin ELISA kit
The Rat Total Bilirubin ELISA kit can be used to identify samples from the rat species. Total Bilirubin can also be called BR, TBIL.
Specifications of Rat Total Bilirubin ELISA kit
Product Information | |
Cat. No. | E02T0143 |
Product Name | Rat Total Bilirubin ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 5-100 umol/L |
Sensitivity | 1.0 umol/L |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 umol/L | 1 vial |
STANDARD B (0.5mL) | 5.0 umol/L | 1 vial |
STANDARD C (0.5mL) | 10 umol/L | 1 vial |
STANDARD D (0.5mL) | 25 umol/L | 1 vial |
STANDARD E (0.5mL) | 50 umol/L | 1 vial |
STANDARD F (0.5mL) | 100 umol/L | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
TBIL ELISA kit uses an anti-ITBIL antibody and an ITBIL-HRP conjugate in a competitive enzyme immunoassay method. ITBIL-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-ITBIL antibody binding site between ITBIL from samples and ITBIL-HRP conjugate, the intensity of the color is inversely proportional to the concentration of ITBIL. Since the number of sites is limited, as more sites are occupied by ITBIL from the sample, fewer sites are left to bind ITBIL-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ITBIL concentration in each sample is interpolated from this standard curve. |
Quality Control on Rat Total Bilirubin ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between TBIL and analogues was observed. | |
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Summary of the Assay Procedure for Rat Total Bilirubin ELISA kit
Citations of Rat Total Bilirubin ELISA kit
E02T0143 has been referenced in the below publications：
Oral administration of oleanolic acid, isolated from Swertia mussotii Franch, attenuates liver injury,inflammation, and cholestasis in bile duct-ligated rats.
Swertianlarin, isolated from Swertia mussotii Franch, increases detoxification enzymes and efflux transporters expression in rats.
