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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Sheep Immunoglobulin G ELISA kit

E14I0058 Sheep Immunoglobulin G ELISA kit

Sheep Immunoglobulin G ELISA kit is suitable for the detection of samples from Sheep species. Immunoglobulin G can also be called Immunoglobulin G, IgG, Ig G.

Specifications of Sheep Immunoglobulin G ELISA kit

Product Information

Cat. No.

E14I0058

Product Name

Sheep Immunoglobulin G ELISA kit

Species

Sheep

Product Size

48 Tests / 96 Tests

Concentration

2.5-50 mg/mL

Sensitivity

0.1 mg/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 mg/mL

1 vial

STANDARD B (0.5mL)

2.5 mg/mL

1 vial

STANDARD C (0.5mL)

5 mg/mL

1 vial

STANDARD D (0.5mL)

10 mg/mL

1 vial

STANDARD E (0.5mL)

25 mg/mL

1 vial

STANDARD F (0.5mL)

50 mg/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

IgG ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-IgG antibody and an IgG-HRP conjugate. The assay sample and buffer are incubated together with IgG-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the IgG concentration since IgG from samples and IgG-HRP conjugate compete for the anti-IgG antibody binding site. Since the number of sites is limited, as more sites are occupied by IgG from the sample, fewer sites are left to bind IgG-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The IgG concentration in each sample is interpolated from this standard curve.


Quality Control on Sheep Immunoglobulin G ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

87-105%

Linearity

Diluent Ratio

Range %

1:2

84-108

1:4

81-107

1:8

80-110

Specificity/Cross-reactivity

No significant cross-reactivity or interference between IgG and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Sheep Immunoglobulin G ELISA kit

Summary of the Assay Procedure for Sheep Immunoglobulin G ELISA kit

Citations of Sheep Immunoglobulin G ELISA kit

E14I0058 has been referenced in the below publications:

Study on the Change of Early Serum and Intestinal Immune Factors in the Small Intestine of Kazak Sheep.

Assessment Model of General Disease Resistance With Principal Component Analysis in Sheeps.

The Comparison of Some Blood Immune Parameters in Sheep.

Effects of α-Lipoic Acid on Growth Performance, Antioxidant and Immunity of Sheep under Heat Stress.

Identification of immune‑responsive genes for adaptative traits by comparative transcriptome analysis of spleen tissue from Kazakh and Sufolk sheep.

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