En
Email
  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Human Nicotinamide phosphoribosyltransferase/ Visfatin ELISA kit (E01V0003)

E01V0003 Human Nicotinamide phosphoribosyltransferase/ Visfatin ELISA kit

The Human Nicotinamide phosphoribosyltransferase/ Visfatin ELISA kit can be used to identify samples from the human species. Nicotinamide phosphoribosyltransferase/ Visfatin can also be called NAmPRTase/NAMPT/VF, Nampt, NAmPRTase, PBEF, PBEF1, Pre-B-Cell Colony Enhancing Factor, Nicotinamide Phosphoribosyltransferase.

Products

Specifications of Human Nicotinamide phosphoribosyltransferase/ Visfatin ELISA kit

Product Information

Cat. No.

E01V0003 

Product Name

Human Nicotinamide phosphoribosyltransferase/ Visfatin ELISA kit

Species

Human

Product Size

48 Tests / 96 Tests

Concentration

50-1000 ng/ml

Sensitivity

1.0 ng/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

50 ng/mL

1 vial

STANDARD C (0.5mL)

100 ng/mL

1 vial

STANDARD D (0.5mL)

250 ng/mL

1 vial

STANDARD E (0.5mL)

500 ng/mL

1 vial

STANDARD F (0.5mL)

1000 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

NAmPRTase/NAMPT/VF ELISA kit uses an anti-NAmPRTase/NAMPT/VF antibody and an NAmPRTase/NAMPT/VF-HRP conjugate in a competitive enzyme immunoassay method. NAmPRTase/NAMPT/VF-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-NAmPRTase/NAMPT/VF antibody binding site between NAmPRTase/NAMPT/VF from samples and NAmPRTase/NAMPT/VF-HRP conjugate, the intensity of the color is inversely proportional to the concentration of NAmPRTase/NAMPT/VF. Since the number of sites is limited, as more sites are occupied by NAmPRTase/NAMPT/VF from the sample, fewer sites are left to bind NAmPRTase/NAMPT/VF-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NAmPRTase/NAMPT/VF concentration in each sample is interpolated from this standard curve.


Quality Control on Human Nicotinamide phosphoribosyltransferase/ Visfatin ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between NAmPRTase/NAMPT/VF and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Human Nicotinamide phosphoribosyltransferase/ Visfatin ELISA kit

Summary of the Assay Procedure for Human Nicotinamide phosphoribosyltransferase/ Visfatin ELISA kit

Citations of Human Nicotinamide phosphoribosyltransferase/ Visfatin ELISA kit

E01V0003 has been referenced in the below publications:

High-density lipoprotein inhibits ox-LDL-induced adipokine secretion by upregulating SR-BI expression and suppressing ER Stress pathway.

Small for gestational age infants plasma visfatin levels and its clinical significance.

Study on the Change of Early Serum and Intestinal Immune Factors in the Small Intestine of Kazak Sheep.

The expression of visfatin and PPARγ in gestational diabetes and normal pregnants.

The clinical study of metabolic disorders and the correlation with Adipocytokines in PCOS women.

Related Bluegene Biotech Products

Join us and become BlueGene's distributor, you will get a broad range of Life science research products and more than 12 years of experienced technical supports.