E11A1169 Bovine Acetyl CoA Carboxylase 1 ELISA kit
Bovine Acetyl CoA carboxylase 1 ELISA kit is suitable for the detection of samples from Bovine species. Acetyl CoA carboxylase 1 can also be called ACAC, ACC1, ACCA, ACAC, ACC2, ACCB, ACACB.
Specifications of Bovine Acetyl CoA Carboxylase 1 ELISA kit
Product Information | |
Cat. No. | E11A1169 |
Product Name | Bovine Acetyl CoA carboxylase 1 ELISA kit |
Species | Bovine |
Product Size | 48 Tests / 96 Tests |
Concentration | 0.5-10 ng/mL |
Sensitivity | 0.1 ng/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 0.5 ng/mL | 1 vial |
STANDARD C (0.5mL) | 1 ng/mL | 1 vial |
STANDARD D (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD E (0.5mL) | 5 ng/mL | 1 vial |
STANDARD F (0.5mL) | 10 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
The competitive enzyme immunoassay method is used by the ACACA ELISA kit in conjunction with an anti-ACACA antibody and an ACACA-HRP conjugate. In a pre-coated plate, the test sample, buffer, and ACACA-HRP conjugate are incubated for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-ACACA antibody binding site between ACACA from samples and ACACA-HRP conjugate, the intensity of the color is inversely proportional to the concentration of ACACA. As more sites are filled by ACACA from the sample, fewer sites are left to bind ACACA-HRP conjugate since the number of sites is restricted. A standard curve is drawn connecting the color's intensity (O.D.) and standard concentration. |
Quality Control on Bovine Acetyl CoA Carboxylase 1 ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 90-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 87-105 | |
1:4 | 84-109 | |
1:8 | 85-107 | |
Specificity/ Cross-reactivity | No significant cross-reactivity or interference between ACACA and analogues was observed. | |
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Summary of the Assay Procedure for Bovine Acetyl CoA Carboxylase 1 ELISA kit
Citations of Bovine Acetyl CoA Carboxylase 1 ELISA kit
E11A1169 has been referenced in the below publications:
Non-Esterified Fatty Acids Activate the AMP-Activated Protein Kinase Signaling Pathway to Regulate Lipid Metabolism in Bovine Hepatocytes.
