Bovine Progesterone ELISA kit - Shanghai BlueGene Biotech CO., LTD.

Specifications of Bovine Progesterone ELISA kit

Product Information
Cat. No.	E11P0200
Product Name	Bovine Progesterone ELISA kit
Species	Bovine
Product Size	48 Tests / 96 Tests
Concentration	2.5-50 ng/mL
Sensitivity	0.1 ng/mL
Principal	Competitive ELISA
Sample Volume	100 ul
Sample Type	Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Time	90 minutes
Platform	Microplate Reader
Conjugate	HRP
Detection Method	Colorimetric
Storage	2-8°C

Kit Components
MATERIALS	SPECIFICATION	QUANTITY
MICROTITER PLATE	96 wells	stripwell
ENZYME CONJUGATE	6.0 mL	1 vial
STANDARD A (0.5mL)	0 ng/mL	1 vial
STANDARD B (0.5mL)	2.5 ng/mL	1 vial
STANDARD C (0.5mL)	5 ng/mL	1 vial
STANDARD D (0.5mL)	10 ng/mL	1 vial
STANDARD E (0.5mL)	25 ng/mL	1 vial
STANDARD F (0.5mL)	50 ng/mL	1 vial
SUBSTRATE A	6 mL	1 vial
SUBSTRATE B	6 mL	1 vial
STOP SOLUTION	6 mL	1 vial
WASH SOLUTION (100 x)	10 mL	1 vial
BALANCE SOLUTION	3 mL	1 vial

Principle of the Assay

PROG ELISA kit uses an anti-PROG antibody and an PROG-HRP conjugate in a competitive enzyme immunoassay method. PROG-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-PROG antibody binding site between PROG from samples and PROG-HRP conjugate, the intensity of the color is inversely proportional to the concentration of PROG. Since the number of sites is limited, as more sites are occupied by PROG from the sample, fewer sites are left to bind PROG-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PROG concentration in each sample is interpolated from this standard curve.

Quality Control on Bovine Progesterone ELISA kit

Coefficient of Variance	Intra Variation% ＜10%
	Inter Variation% ＜12%
Recovery	95-102%
Linearity	Diluent Ratio	Range %
	1:2	93-105
	1:4	88-106
	1:8	86-108
Specificity/Cross-reactivity	No significant cross-reactivity or interference between PROG and analogues was observed.

BlueGene Biotech Product Show

Summary of the Assay Procedure for Bovine Progesterone ELISA kit

Citations of Bovine Progesterone ELISA kit

E11P0200 has been referenced in the below publications：

impact of fertility-promoting powder on trace elements and hormone in serum of dairy cattle with ovarian static disease.

A Study on the Impact of Reproductive Hormones in Serum by Using Fertility-Promoting Powder for Treating Infertility Caused by Persistent Corpus Luteum and Ovary Sandstill of Dairy Cattle.

study on the impact of fertility-promoting powder on reproductive hormones in serum of dairy cattle with persistent corpus luteum.

Study on Effect of Reproductive Performance of Cows on Biotin and Endocrine Mechanism.

Study on the Dagnosis Ovarian Disease by B-mode Ultrasound Scanner and Optimization of the Treatment Protocol on Different Stage of Postpartum in Dairy Cows.

奶牛排卵延迟的综合诊断。

中药复方理囊散对奶牛子宫复旧期间生殖激素的影响。

The correlation analysis of trace elements and hormones in serum of infertile dairy cattle with ovarian stationary treated with fertility-promoting powder.

Correlation Analysis of IL and Hormone in Serum During Treatment of Persistent Courpus Luteum with Fertility-promoting Power in Dairy Cows.

Rule Analysis of LH，E2 and P4 of Ovulation-Delayed Dairy Cows at Different Stages after Estrus.

奶牛卵巢囊肿的综合诊断。