En
Email
  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Chicken 72 kDa type IV collagenase ELISA kit

E12M0445 Chicken 72 kDa type IV collagenase ELISA kit

Chicken 72 kDa type IV collagenase ELISA kit is suitable for the detection of samples from Chicken species. 72 kDa type IV collagenase can also be called MMP2, MMP 2, 72 kDa gelatinase, 72kD type IV collagenase, CLG 4, CLG 4A, CLG4, CLG4A, Collagenase Type 4 alpha, Collagenase type IV A, Gelatinase A, Gelatinase alpha, Gelatinase neutrophil.

Specifications of Chicken 72 kDa type IV collagenase ELISA kit

Product Information

Cat. No.

E12M0445

Product Name

Chicken 72 kDa type IV collagenase ELISA kit

Species

Chicken

Product Size

48 Tests / 96 Tests

Concentration

5.0-100 ng/mL

Sensitivity

1.0 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

5 ng/mL

1 vial

STANDARD C (0.5mL)

10 ng/mL

1 vial

STANDARD D (0.5mL)

25 ng/mL

1 vial

STANDARD E (0.5mL)

50 ng/mL

1 vial

STANDARD F (0.5mL)

100 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

MMP2 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-MMP2 antibody and an MMP2-HRP conjugate. The assay sample and buffer are incubated together with MMP2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MMP2 concentration since MMP2 from samples and MMP2-HRP conjugate compete for the anti-MMP2 antibody binding site. Since the number of sites is limited, as more sites are occupied by MMP2 from the sample, fewer sites are left to bind MMP2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MMP2 concentration in each sample is interpolated from this standard curve.


Quality Control on Chicken 72 kDa type IV collagenase ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

91-105%

Linearity

Diluent Ratio

Range %

1:2

88-106

1:4

87-108

1:8

85-110

Specificity/Cross-reactivity

No significant cross-reactivity or interference between MMP2 and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Chicken 72 kDa type IV collagenase ELISA kit

Summary of the Assay Procedure for Chicken 72 kDa type IV collagenase ELISA kit

Citations of Chicken 72 kDa type IV collagenase ELISA kit

E12M0445 has been referenced in the below publications:

Roles of miRNA and MDV oncogene Meq in the transformation of chicken Marek's disease lymphoma.


Related Bluegene Biotech Products

Join us and become BlueGene's distributor, you will get a broad range of Life science research products and more than 12 years of experienced technical supports.