En
Email
  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Chicken Free cholesterol ELISA kit (E12F0212)

E12F0212 Chicken Free cholesterol ELISA kit

Chicken Free cholesterol ELISA kit is suitable for the detection of samples from Chicken species. Free cholesterol can also be called Free 3β-Hydroxy-5-cholestene, Free 5-Cholesten-3β, FCHOL.

Products

Specifications of Chicken Free cholesterol ELISA kit

Product Information

Cat. No.

E12F0212

Product Name

Chicken Free cholesterol ELISA kit

Species

Chicken

Product Size

48 Tests / 96 Tests

Concentration

5.0-100 ng/mL

Sensitivity

1.0 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

5 ng/mL

1 vial

STANDARD C (0.5mL)

10 ng/mL

1 vial

STANDARD D (0.5mL)

25 ng/mL

1 vial

STANDARD E (0.5mL)

50 ng/mL

1 vial

STANDARD F (0.5mL)

100 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

FCHOL ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-FCHOL antibody and an FCHOL-HRP conjugate. The assay sample and buffer are incubated together with FCHOL-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the FCHOL concentration since FCHOL from samples and FCHOL-HRP conjugate compete for the anti-FCHOL antibody binding site. Since the number of sites is limited, as more sites are occupied by FCHOL from the sample, fewer sites are left to bind FCHOL-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FCHOL concentration in each sample is interpolated from this standard curve.


Quality Control on Chicken Free cholesterol ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

91-105%

Linearity

Diluent Ratio

Range %

1:2

88-106

1:4

87-108

1:8

85-110

Specificity/Cross-reactivity

No significant cross-reactivity or interference between FCHOL and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Chicken Free cholesterol ELISA kit

Summary of the Assay Procedure for Chicken Free cholesterol ELISA kit

Citations of Chicken Free cholesterol ELISA kit

E12F0212 has been referenced in the below publications:

Impact of Chronic Levels of Naturally MultiContaminated Feed with Fusarium Mycotoxins on Broiler Chickens and Evaluation of the Mitigation Properties of Different Titers of Yeast Cell Wall Extract.


Related Bluegene Biotech Products

Join us and become BlueGene's distributor, you will get a broad range of Life science research products and more than 12 years of experienced technical supports.