E12F0212 Chicken Free cholesterol ELISA kit
Chicken Free cholesterol ELISA kit is suitable for the detection of samples from Chicken species. Free cholesterol can also be called Free 3β-Hydroxy-5-cholestene, Free 5-Cholesten-3β, FCHOL.
Specifications of Chicken Free cholesterol ELISA kit
Product Information | |
Cat. No. | E12F0212 |
Product Name | Chicken Free cholesterol ELISA kit |
Species | Chicken |
Product Size | 48 Tests / 96 Tests |
Concentration | 5.0-100 ng/mL |
Sensitivity | 1.0 ng/mL |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 5 ng/mL | 1 vial |
STANDARD C (0.5mL) | 10 ng/mL | 1 vial |
STANDARD D (0.5mL) | 25 ng/mL | 1 vial |
STANDARD E (0.5mL) | 50 ng/mL | 1 vial |
STANDARD F (0.5mL) | 100 ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
FCHOL ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-FCHOL antibody and an FCHOL-HRP conjugate. The assay sample and buffer are incubated together with FCHOL-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the FCHOL concentration since FCHOL from samples and FCHOL-HRP conjugate compete for the anti-FCHOL antibody binding site. Since the number of sites is limited, as more sites are occupied by FCHOL from the sample, fewer sites are left to bind FCHOL-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The FCHOL concentration in each sample is interpolated from this standard curve. |
Quality Control on Chicken Free cholesterol ELISA kit
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 91-105% | |
Linearity | Diluent Ratio | Range % |
1:2 | 88-106 | |
1:4 | 87-108 | |
1:8 | 85-110 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between FCHOL and analogues was observed. | |
BlueGene Biotech Product Show
Summary of the Assay Procedure for Chicken Free cholesterol ELISA kit
Citations of Chicken Free cholesterol ELISA kit
E12F0212 has been referenced in the below publications:
Impact of Chronic Levels of Naturally MultiContaminated Feed with Fusarium Mycotoxins on Broiler Chickens and Evaluation of the Mitigation Properties of Different Titers of Yeast Cell Wall Extract.
