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BlueGene Biotech Chicken Serotonin Transporter ELISA kit

E12S0089 Chicken Serotonin Transporter ELISA kit

Chicken Serotonin Transporter ELISA kit is suitable for the detection of samples from Chicken species. 7Serotonin Transporter can also be called 5-HTT, 5HTT, HTT, OCD1, SERT, SERT1, hSERT, Solute Carrier Family 6 (neurotransmitter transporter), member 4, solute carrier family 6 member 4, 5-HTTLPR.

Specifications of Chicken Serotonin Transporter ELISA kit

Product Information

Cat. No.

E12S0089

Product Name

Chicken Serotonin Transporter ELISA kit

Species

Chicken

Product Size

48 Tests / 96 Tests

Concentration

5.0-100 ng/mL

Sensitivity

1.0 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

5 ng/mL

1 vial

STANDARD C (0.5mL)

10 ng/mL

1 vial

STANDARD D (0.5mL)

25 ng/mL

1 vial

STANDARD E (0.5mL)

50 ng/mL

1 vial

STANDARD F (0.5mL)

100 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

SERT ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-SERT antibody and an SERT-HRP conjugate. The assay sample and buffer are incubated together with SERT-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SERT concentration since SERT from samples and SERT-HRP conjugate compete for the anti-SERT antibody binding site. Since the number of sites is limited, as more sites are occupied by SERT from the sample, fewer sites are left to bind SERT-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SERT concentration in each sample is interpolated from this standard curve.


Quality Control on Chicken Serotonin Transporter ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

91-105%

Linearity

Diluent Ratio

Range %

1:2

88-106

1:4

87-108

1:8

85-110

Specificity/Cross-reactivity

No significant cross-reactivity or interference between SERT and analogues was observed.


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Summary of the Assay Procedure for Chicken Serotonin Transporter ELISA kit

Summary of the Assay Procedure for Chicken Serotonin Transporter ELISA kit

Citations of Chicken Serotonin Transporter ELISA kit

E12S0089 has been referenced in the below publications:

A functional variant in the 5′flanking region of the chicken serotonin transporter gene is associated with increased body weight and locomotor activity.


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