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  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Rat C1q/TNF related protein 3,Cartonectin/CTRP3/CORS 26 ELISA kit (E02C1243)

E02C1243 Rat C1q/TNF related protein 3,Cartonectin/CTRP3/CORS 26 ELISA kit

The Rat C1q/TNF related protein 3,Cartonectin/CTRP3/CORS 26 ELISA kit can be used to identify samples from the rat species. C1q/TNF related protein 3,Cartonectin/CTRP3/CORS 26 can also be called CORS; CORCS; CTRP3; CORS26; C1ATNF3; CORS-26.

Products

Specifications of Rat C1q/TNF related protein 3,Cartonectin/CTRP3/CORS 26 ELISA kit

Product Information

Cat. No.

E02C1243

Product Name

Rat C1q/TNF related protein 3,Cartonectin/CTRP3/CORS 26 ELISA kit

Species

Rat

Product Size

48 Tests / 96 Tests

Concentration

1.0-25 ng/mL

Sensitivity

0.1ng/ml

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

1.0 ng/mL

1 vial

STANDARD C (0.5mL)

2.5 ng/mL

1 vial

STANDARD D (0.5mL)

5.0 ng/mL

1 vial

STANDARD E (0.5mL)

10 ng/mL

1 vial

STANDARD F (0.5mL)

25ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

C1QTNF3 ELISA kit uses an anti-C1QTNF3 antibody and an C1QTNF3-HRP conjugate in a competitive enzyme immunoassay method. C1QTNF3-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-C1QTNF3 antibody binding site between C1QTNF3 from samples and C1QTNF3-HRP conjugate, the intensity of the color is inversely proportional to the concentration of C1QTNF3. Since the number of sites is limited, as more sites are occupied by C1QTNF3 from the sample, fewer sites are left to bind C1QTNF3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The C1QTNF3 concentration in each sample is interpolated from this standard curve.


Quality Control on Rat C1q/TNF related protein 3,Cartonectin/CTRP3/CORS 26 ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

95-102%

Linearity

Diluent Ratio

Range %

1:2

93-105

1:4

88-106

1:8

86-108

Specificity/Cross-reactivity

No significant cross-reactivity or interference between C1QTNF3 and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Rat C1q/TNF related protein 3,Cartonectin/CTRP3/CORS 26 ELISA kit

Summary of the Assay Procedure for Rat C1q/TNF related protein 3,Cartonectin/CTRP3/CORS 26 ELISA kit

Citations of Rat C1q/TNF related protein 3,Cartonectin/CTRP3/CORS 26 ELISA kit

E02C1243 has been referenced in the below publications:

Effects of chronic ethanol consumption on levels of adipokines in visceral adipose tissues and sera of rats.

EFFECTS OF DIET (ETHANOL CONSUMPTION AND HIGH-FAT CONSUMPTION) ON LEVELS OF ADIPOKINES IN VISCERAL ADIPOSE TISSUES AND SERA OF RATS.


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