E02C1243 Rat C1q/TNF related protein 3,Cartonectin/CTRP3/CORS 26 ELISA kit
The Rat C1q/TNF related protein 3,Cartonectin/CTRP3/CORS 26 ELISA kit can be used to identify samples from the rat species. C1q/TNF related protein 3,Cartonectin/CTRP3/CORS 26 can also be called CORS; CORCS; CTRP3; CORS26; C1ATNF3; CORS-26.
Product Information | |
Cat. No. | E02C1243 |
Product Name | Rat C1q/TNF related protein 3,Cartonectin/CTRP3/CORS 26 ELISA kit |
Species | Rat |
Product Size | 48 Tests / 96 Tests |
Concentration | 1.0-25 ng/mL |
Sensitivity | 0.1ng/ml |
Principal | Competitive ELISA |
Sample Volume | 100 ul |
Sample Type | Serum, plasma, cell culture supernatants, body fluid and tissue homogenate |
Assay Time | 90 minutes |
Platform | Microplate Reader |
Conjugate | HRP |
Detection Method | Colorimetric |
Storage | 2-8°C |
Kit Components | ||
MATERIALS | SPECIFICATION | QUANTITY |
MICROTITER PLATE | 96 wells | stripwell |
ENZYME CONJUGATE | 6.0 mL | 1 vial |
STANDARD A (0.5mL) | 0 ng/mL | 1 vial |
STANDARD B (0.5mL) | 1.0 ng/mL | 1 vial |
STANDARD C (0.5mL) | 2.5 ng/mL | 1 vial |
STANDARD D (0.5mL) | 5.0 ng/mL | 1 vial |
STANDARD E (0.5mL) | 10 ng/mL | 1 vial |
STANDARD F (0.5mL) | 25ng/mL | 1 vial |
SUBSTRATE A | 6 mL | 1 vial |
SUBSTRATE B | 6 mL | 1 vial |
STOP SOLUTION | 6 mL | 1 vial |
WASH SOLUTION (100 x) | 10 mL | 1 vial |
BALANCE SOLUTION | 3 mL | 1 vial |
Principle of the Assay |
C1QTNF3 ELISA kit uses an anti-C1QTNF3 antibody and an C1QTNF3-HRP conjugate in a competitive enzyme immunoassay method. C1QTNF3-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-C1QTNF3 antibody binding site between C1QTNF3 from samples and C1QTNF3-HRP conjugate, the intensity of the color is inversely proportional to the concentration of C1QTNF3. Since the number of sites is limited, as more sites are occupied by C1QTNF3 from the sample, fewer sites are left to bind C1QTNF3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The C1QTNF3 concentration in each sample is interpolated from this standard curve. |
Coefficient of Variance | Intra Variation% ＜10% | |
Inter Variation% ＜12% | ||
Recovery | 95-102% | |
Linearity | Diluent Ratio | Range % |
1:2 | 93-105 | |
1:4 | 88-106 | |
1:8 | 86-108 | |
Specificity/Cross-reactivity | No significant cross-reactivity or interference between C1QTNF3 and analogues was observed. | |
E02C1243 has been referenced in the below publications：
Effects of chronic ethanol consumption on levels of adipokines in visceral adipose tissues and sera of rats.
EFFECTS OF DIET (ETHANOL CONSUMPTION AND HIGH-FAT CONSUMPTION) ON LEVELS OF ADIPOKINES IN VISCERAL ADIPOSE TISSUES AND SERA OF RATS.
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