En
Email
  • bovine serum albumin elisa 1
  • bovine serum albumin elisa 1

BlueGene Biotech Porcine Aggrecan ELISA kit

E07A0413 Porcine Aggrecan ELISA kit

Porcine Aggrecan ELISA kit is suitable for detecting samples from porcine species. Aggrecan can also be called cartilage-specific proteoglycan core protein, CSPCP, chondroitin sulfate proteoglycan 1, ACAN, AGC1, AGCAN, CSPG1, CSPGCP, MSK16, SEDK, SSOAOD.

Specifications of Porcine Aggrecan ELISA kit

Product Information

Cat. No.

E07A0413

Product Name

Porcine Aggrecan ELISA kit

Species

Porcine

Product Size

48 Tests / 96 Tests

Concentration

5-100 ng/mL

Sensitivity

1.0 ng/mL

Principal

Competitive ELISA

Sample Volume

100 ul

Sample Type

Serum, plasma, cell culture supernatants, body fluid and tissue homogenate

Assay Time

90 minutes

Platform

Microplate Reader

Conjugate

HRP

Detection Method

Colorimetric

Storage

2-8°C


Kit Components

MATERIALS

SPECIFICATION

QUANTITY

MICROTITER PLATE

96 wells

stripwell

ENZYME CONJUGATE

6.0 mL

1 vial

STANDARD A (0.5mL)

0 ng/mL

1 vial

STANDARD B (0.5mL)

5.0 ng/mL

1 vial

STANDARD C (0.5mL)

10 ng/mL

1 vial

STANDARD D (0.5mL)

25 ng/mL

1 vial

STANDARD E (0.5mL)

50 ng/mL

1 vial

STANDARD F (0.5mL)

100 ng/mL

1 vial

SUBSTRATE A

6 mL

1 vial

SUBSTRATE B

6 mL

1 vial

STOP SOLUTION

6 mL

1 vial

WASH SOLUTION (100 x)

10 mL

1 vial

BALANCE SOLUTION

3 mL

1 vial


Principle of the Assay

AGC ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-AGC antibody and an AGC-HRP conjugate. The assay sample and buffer are incubated together with AGC-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AGC concentration since AGC from samples and AGC-HRP conjugate compete for the anti-AGC antibody binding site. Since the number of sites is limited, as more sites are occupied by AGC from the sample, fewer sites are left to bind AGC-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AGC concentration in each sample is interpolated from this standard curve.


Quality Control on Porcine Aggrecan ELISA kit

Coefficient of Variance

Intra Variation% <10%

Inter Variation% <12%

Recovery

94-107%

Linearity

Diluent Ratio

Range %

1:2

95-112

1:4

96-108

1:8

91-103

Specificity/Cross-reactivity

No significant cross-reactivity or interference between AGC and analogues was observed.


BlueGene Biotech Product Show

Summary of the Assay Procedure for Porcine Aggrecan ELISA kit

Summary of the Assay Procedure for Porcine Aggrecan ELISA kit

Citations of Porcine Aggrecan ELISA kit

E07A0413 has been referenced in the below publications:

Increased lactic acid content associated with extracellular matrix depletion in a porcine disc degeneration induced by superficial annular lesion.

Related Bluegene Biotech Products

Join us and become BlueGene's distributor, you will get a broad range of Life science research products and more than 12 years of experienced technical supports.